Confirmation of First Report of Orchid fleck virus in Phalaenopsis Hybrid Orchids in the USA

Sara A Bratsch, B. E. Lockhart, Carol A Ishimaru

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Orchidaceae family members are diverse and are used for food, cut flowers, and potted plants. In 2013, the United States sold $246 million of potted orchids and $5.6 million of cut flower orchids. Sixteen Phalaenopsis hybrid orchids were submitted for pathogen identification in December 2014. New leaves exhibited necrotic flecks with chlorotic rings (Fig. 1 A) or necrotic lesions with leaf reddening (Fig. 1 B). Older leaves on the same plants were either asymptomatic or had large chlorotic spots. Flowers were asymptomatic (Fig. 1 C). Bacterial streaming was not observed under low magnification and very few bacterial colonies on nutrient broth yeast extract medium were isolated from symptomatic tissues. Serological immunostrip testing for Cymbidium mosaic virus and Odontoglossum ringspot virus were negative (Agdia Inc., Elkhart, IN). Brevipalpus sp. mites were observed by dissecting microscopy on several leaves. Transmission electron microscopy (TEM) of symptomatic leaves using partially purified extracts revealed no virus particles. TEM using a rhabdovirus extraction protocol (Doi et al. 1977) confirmed the presence of bacilliform virus particles. One characterized orchid-infecting bacilliform virus is Orchid fleck virus (OFV) (Peng et al. 2013)

Original languageEnglish (US)
Pages (from-to)146-148
Number of pages3
JournalPlant Health Progress
Volume16
Issue number4
DOIs
StatePublished - 2015

Bibliographical note

Funding Information:
The authors would like to thank Dr. Nancy J. Ehlke, Department of Agronomy and Plant Genetics at the University of Minnesota, for assistance with plant materials

Publisher Copyright:
© 2015 The American Phytopathological Society

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