Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system

Nicholas J. Baltes, Aaron W. Hummel, Eva Konecna, Radim Cegan, Aaron N. Bruns, David M. Bisaro, Daniel F. Voytas

Research output: Contribution to journalArticlepeer-review

142 Scopus citations


To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.

Original languageEnglish (US)
Article number15145
JournalNature plants
Issue number10
StatePublished - Oct 2015

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