The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
|Original language||English (US)|
|State||Published - Nov 15 2018|
Bibliographical noteFunding Information:
This work was supported by grants from the National Transgenic Science and Technology Program (2016ZX08010-002, 2018ZX0800102B-001 and 2018ZX0801002B-002), the National Natural Science Foundation of China (31788103), and the Chinese Academy of Sciences (QYZDY-SSW-SMC030 and GJHZ1602).
© 2018 The Author(s).
- CRISPR/Cas9 system