Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system

Xiang Ji, Xiaomin Si, Yi Zhang, Huawei Zhang, Feng Zhang, Caixia Gao

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.

Original languageEnglish (US)
Article number197
JournalGenome biology
Volume19
Issue number1
DOIs
StatePublished - Nov 15 2018

Bibliographical note

Funding Information:
This work was supported by grants from the National Transgenic Science and Technology Program (2016ZX08010-002, 2018ZX0800102B-001 and 2018ZX0801002B-002), the National Natural Science Foundation of China (31788103), and the Chinese Academy of Sciences (QYZDY-SSW-SMC030 and GJHZ1602).

Publisher Copyright:
© 2018 The Author(s).

Keywords

  • CRISPR/Cas9 system
  • Geminivirus
  • Off-target
  • Virus-inducible
  • Virus-resistance

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