Conduction-Dominated Cryomesh for Organism Vitrification

Zongqi Guo, Nikolas Zuchowicz, Jessica Bouwmeester, Amey S. Joshi, Amanda L. Neisch, Kieran Smith, Jonathan Daly, Michael L. Etheridge, Erik B. Finger, Suhasa B. Kodandaramaiah, Thomas S. Hays, Mary Hagedorn, John C. Bischof

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Vitrification-based cryopreservation is a promising approach to achieving long-term storage of biological systems for maintaining biodiversity, healthcare, and sustainable food production. Using the “cryomesh” system achieves rapid cooling and rewarming of biomaterials, but further improvement in cooling rates is needed to increase biosystem viability and the ability to cryopreserve new biosystems. Improved cooling rates and viability are possible by enabling conductive cooling through cryomesh. Conduction-dominated cryomesh improves cooling rates from twofold to tenfold (i.e., 0.24 to 1.2 × 105 °C min−1) in a variety of biosystems. Higher thermal conductivity, smaller mesh wire diameter and pore size, and minimizing the nitrogen vapor barrier (e.g., vertical plunging in liquid nitrogen) are key parameters to achieving improved vitrification. Conduction-dominated cryomesh successfully vitrifies coral larvae, Drosophila embryos, and zebrafish embryos with improved outcomes. Not only a theoretical foundation for improved vitrification in µm to mm biosystems but also the capability to scale up for biorepositories and/or agricultural, aquaculture, or scientific use are demonstrated.

Original languageEnglish (US)
Article number2303317
JournalAdvanced Science
Issue number3
StatePublished - Jan 19 2024

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© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.


  • Drosophila embryo
  • coral larvae
  • cryomesh vitrification
  • cryopreservation
  • heat transfer
  • zebrafish embryo


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