Conditional reprogramming culture conditions facilitate growth of lower-grade glioma models

Ming Yuan, David White, Linda Resar, Eli Bar, Mari Groves, Alan Cohen, Eric Jackson, Jennifer Bynum, Jeffrey Rubens, Jeff Mumm, Liam Chen, Liqun Jiang, Eric Raabe, Fausto J. Rodriguez, Charles G. Eberhart

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Background: The conditional reprogramming cell culture method was developed to facilitate growth of senescence-prone normal and neoplastic epithelial cells, and involves co-culture with irradiated fibroblasts and the addition of a small molecule Rho kinase (ROCK) inhibitor. The aim of this study was to determine whether this approach would facilitate the culture of compact low-grade gliomas. Methods: We attempted to culture 4 pilocytic astrocytomas, 2 gangliogliomas, 2 myxopapillary ependymomas, 2 anaplastic gliomas, 2 difficult-to-classify low-grade neuroepithelial tumors, a desmoplastic infantile ganglioglioma, and an anaplastic pleomorphic xanthoastrocytoma using a modified conditional reprogramming cell culture approach. Results: Conditional reprogramming resulted in robust increases in growth for a majority of these tumors, with fibroblast conditioned media and ROCK inhibition both required. Switching cultures to standard serum containing media, or serum-free neurosphere conditions, with or without ROCK inhibition, resulted in decreased proliferation and induction of senescence markers. Rho kinase inhibition and conditioned media both promoted Akt and Erk1/2 activation. Several cultures, including one derived from a NF1-associated pilocytic astrocytoma (JHH-NF1-PA1) and one from a BRAF p.V600E mutant anaplastic pleomorphic xanthoastrocytoma (JHH-PXA1), exhibited growth sufficient for preclinical testing in vitro. In addition, JHH-NF1-PA1 cells survived and migrated in larval zebrafish orthotopic xenografts, while JHH-PXA1 formed orthotopic xenografts in mice histopathologically similar to the tumor from which it was derived. Conclusions: These studies highlight the potential for the conditional reprogramming cell culture method to promote the growth of glial and glioneuronal tumors in vitro, in some cases enabling the establishment of long-term culture and in vivo models.

Original languageEnglish (US)
Pages (from-to)770-782
Number of pages13
Issue number5
StatePublished - Dec 1 2020

Bibliographical note

Publisher Copyright:
© 2020 The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved.


  • BRAFV600E
  • NF1
  • conditional reprogramming
  • enescence
  • low-grade glioma


Dive into the research topics of 'Conditional reprogramming culture conditions facilitate growth of lower-grade glioma models'. Together they form a unique fingerprint.

Cite this