Concise Review: Human-Animal Neurological Chimeras: Humanized Animals or Human Cells in an Animal?

Andrew T. Crane, Joseph P. Voth, Francis X. Shen, Walter C. Low

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Blastocyst complementation is an emerging methodology in which human stem cells are transferred into genetically engineered preimplantation animal embryos eventually giving rise to fully developed human tissues and organs within the animal host for use in regenerative medicine. The ethical issues surrounding this method have caused the National Institutes of Health to issue a moratorium on funding for blastocyst complementation citing the potential for human cells to substantially contribute to the brain of the chimeric animal. To address this concern, we performed an in-depth review of the neural transplantation literature to determine how the integration of human cells into the nonhuman neural circuitry has altered the behavior of the host. Despite reports of widespread integration of human cell transplants, our review of 150 transplantation studies found no evidence suggestive of humanization of the animal host, and we thus conclude that, at present, concerns over humanization should not prevent research on blastocyst complementation to continue. We suggest proceeding in a controlled and transparent manner, however, and include recommendations for future research with careful consideration for how human cells may contribute to the animal host nervous system. Stem Cells 2019;37:444–452.

Original languageEnglish (US)
Pages (from-to)444-452
Number of pages9
JournalSTEM CELLS
Volume37
Issue number4
DOIs
StatePublished - Apr 2019

Fingerprint

Blastocyst
Stem Cells
Transplantation
Animal Structures
Genetically Modified Animals
Regenerative Medicine
National Institutes of Health (U.S.)
Ethics
Nervous System
Transplants
Brain
Research

Keywords

  • Behavior
  • Blastocyst complementation
  • Cell transplantation
  • Chimera
  • Cognition
  • Stem cells

Cite this

Concise Review : Human-Animal Neurological Chimeras: Humanized Animals or Human Cells in an Animal? / Crane, Andrew T.; Voth, Joseph P.; Shen, Francis X.; Low, Walter C.

In: STEM CELLS, Vol. 37, No. 4, 04.2019, p. 444-452.

Research output: Contribution to journalReview article

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