Concentration control for protein crystallization via a continuously-fed crystallization chamber

Masano Sugiyama, Shramik Sengupta, Paul Todd, Victor H. Barocas

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A continuously-fed crystallization chamber that allows for kinetic path control through the crystallization phase diagram (from labile/nucleation to metastable/growth) was fabricated and used to crystallize lysozyme. A lumped kinetic model was developed, and parameters for heterogeneous nucleation kinetics were determined. Heterogeneous nucleation was found to have faster nucleation kinetics and slower growth kinetics than homogeneous nucleation, as expected. The major contributions of the new device are (1) to allow better control of the chemical environment for studies of crystal nucleation and growth, and (2) to allow lumped-model analysis of those studies to extract kinetic parameters.

Original languageEnglish (US)
Pages (from-to)1398-1401
Number of pages4
JournalLab on a chip
Volume8
Issue number8
DOIs
StatePublished - 2008

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