Objective - To provide a detailed description of cross-sectional anatomic structures of the manus in canine cadavers in association with corresponding features in computed tomographic (CT) and magnetic resonance (MR) images. Sample Population - 7 cadavers of adult large-breed-type dogs (weight range, 25 to 30 kg) without forelimb disease. Procedures - Forelimbs were removed from the cadavers within 4 hours after euthanasia and frozen. The right forelimbs of 3 cadavers were cut into 4-mm sections by use of a band saw; 1 limb each was sectioned in the transverse, dorsal, or sagittal plane. Sections were cleaned and then photographed. After thawing, transverse CT images of the right forelimbs of 3 additional cadavers were obtained, and the right forelimb of a seventh cadaver underwent MR imaging in the transverse, sagittal, and dorsal planes. The evaluated regions extended from the digits to the carpus. Features in CT and MR images that corresponded to clinically important anatomic structures in tissue sections were identified. Results - For most of the anatomic structures evident in tissue sections, corresponding CT and MR imaging features were identified. Osseous and musculotendinous structures of the manus were readily detected in CT and MR images, whereas vascular structures were only rarely identified by use of the imaging techniques. Conclusions and Clinical Relevance - Results of the detailed assessment of anatomic structures of the canine manus in association with corresponding features in CT and MR images will facilitate detection of pathological conditions and be beneficial in planning surgical procedures for diseases of the manus in dogs.