Comprehensive Analysis of DNA Adducts Using Data-Independent wSIM/MS2Acquisition and wSIM-City

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A novel software has been created to comprehensively characterize covalent modifications of DNA through mass spectral analysis of enzymatically hydrolyzed DNA using the neutral loss of 2′-deoxyribose, a nearly universal MS2 fragmentation process of protonated 2′-deoxyribonucleosides. These covalent modifications termed DNA adducts form through xenobiotic exposures or by reaction with endogenous electrophiles and can induce mutations during cell division and initiate carcinogenesis. DNA adducts are typically present at trace levels in the human genome, requiring a very sensitive and comprehensive data acquisition and analysis method. Our software, wSIM-City, was created to process mass spectral data acquired by a wide selected ion monitoring (wSIM) with gas-phase fractionation and coupled to wide MS2 fragmentation. This untargeted approach can detect DNA adducts at trace levels as low as 1.5 adducts per 109 nucleotides. This level of sensitivity is sufficient for comprehensive analysis and characterization of DNA modifications in human specimens.

Original languageEnglish (US)
Pages (from-to)6491-6500
Number of pages10
JournalAnalytical Chemistry
Volume93
Issue number16
DOIs
StatePublished - Apr 27 2021

Bibliographical note

Publisher Copyright:
© 2021 American Chemical Society.

Fingerprint

Dive into the research topics of 'Comprehensive Analysis of DNA Adducts Using Data-Independent wSIM/MS2Acquisition and wSIM-City'. Together they form a unique fingerprint.

Cite this