TY - JOUR
T1 - Composition of peripheral blood progenitor cell components collected from healthy donors
AU - Stroncek, D. F.
AU - Clay, M. E.
AU - Smith, J.
AU - Herr, G.
AU - Ilstrup, S.
AU - Kunkel, L. A.
AU - Mc Cullough, Jeffrey
PY - 1997/4
Y1 - 1997/4
N2 - BACKGROUND: Peripheral blood progenitor cell (PBPC) components are being collected from healthy donors for allogeneic transplantation, but the quantity, quality, composition, and variability of PBPCs collected from healthy people given granulocyte-colony-stimulating factor (G-CSF) have not been evaluated. STUDY DESIGN AND METHODS: PBPC components were collected from 150 healthy people who were given G-CSF (5, 7.5, or 10 μg/kg/day) for 5 days. The components were evaluated for white cell (WBC), mononuclear cell, CD34+ cell, neutrophil, platelet, and red cell (RBC) composition. RESULTS: The quantities collected were: WBCs, 35.0 ± 16.4 x 109 (range, 11.9-163.3 x 109); mononuclear cells, 33.3 ± 14.4 x 109 (range, 11.9-139.6 x 109); CD34+ cells, 412 ± 287 x 106 (range, 70-1658 x 106); neutrophils, 1.71 ± 3.59 x 109 (range, 0-27.6 x 109); RBCs, 7.2 ± 4.0 mL (range, 0-22.1 mL); and platelets, 480 ± 110 x 109 (range, 250-920 x 109). PBPC components collected from people given G-CSF at 7.5 or 10 μg per kg per day contained significantly more CD34+ cells (respectively, 426 ± 300 x 106; range, 70- 1658 x 106 and 452 ± 294 x 106; range, 78-1380 x 106) than those from people given G-CSF at 5 μg per kg per day (276 ± 186 x 106; range, 91-767 x 106) (p = 0.007 and p = 0.002). When 10 μg per kg per day of G-CSF was given, 50 percent of the components contained enough CD34+ cells for transplantation to a 75-kg recipient (375 x 106 CD34+ cells), but 10.6 percent of the components contained less than 150 x 106 CD34+ cells and thus would provide a transplantable dose only for a 30-kg patient. CONCLUSION: One PBPC component collected from a healthy donor given 7.5 or 10 μg per kg per day of G-CSF should contain 70 to 1660 x 106 CD34+ cells, with 0 to 22 mL of RBCs. Because of the great variability in the number of CD34+ cells collected, the quantity of CD34+ cells in each component should be measured after each procedure to ensure that sufficient quantities of cells are present for a successful transplant.
AB - BACKGROUND: Peripheral blood progenitor cell (PBPC) components are being collected from healthy donors for allogeneic transplantation, but the quantity, quality, composition, and variability of PBPCs collected from healthy people given granulocyte-colony-stimulating factor (G-CSF) have not been evaluated. STUDY DESIGN AND METHODS: PBPC components were collected from 150 healthy people who were given G-CSF (5, 7.5, or 10 μg/kg/day) for 5 days. The components were evaluated for white cell (WBC), mononuclear cell, CD34+ cell, neutrophil, platelet, and red cell (RBC) composition. RESULTS: The quantities collected were: WBCs, 35.0 ± 16.4 x 109 (range, 11.9-163.3 x 109); mononuclear cells, 33.3 ± 14.4 x 109 (range, 11.9-139.6 x 109); CD34+ cells, 412 ± 287 x 106 (range, 70-1658 x 106); neutrophils, 1.71 ± 3.59 x 109 (range, 0-27.6 x 109); RBCs, 7.2 ± 4.0 mL (range, 0-22.1 mL); and platelets, 480 ± 110 x 109 (range, 250-920 x 109). PBPC components collected from people given G-CSF at 7.5 or 10 μg per kg per day contained significantly more CD34+ cells (respectively, 426 ± 300 x 106; range, 70- 1658 x 106 and 452 ± 294 x 106; range, 78-1380 x 106) than those from people given G-CSF at 5 μg per kg per day (276 ± 186 x 106; range, 91-767 x 106) (p = 0.007 and p = 0.002). When 10 μg per kg per day of G-CSF was given, 50 percent of the components contained enough CD34+ cells for transplantation to a 75-kg recipient (375 x 106 CD34+ cells), but 10.6 percent of the components contained less than 150 x 106 CD34+ cells and thus would provide a transplantable dose only for a 30-kg patient. CONCLUSION: One PBPC component collected from a healthy donor given 7.5 or 10 μg per kg per day of G-CSF should contain 70 to 1660 x 106 CD34+ cells, with 0 to 22 mL of RBCs. Because of the great variability in the number of CD34+ cells collected, the quantity of CD34+ cells in each component should be measured after each procedure to ensure that sufficient quantities of cells are present for a successful transplant.
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U2 - 10.1046/j.1537-2995.1997.37497265342.x
DO - 10.1046/j.1537-2995.1997.37497265342.x
M3 - Article
C2 - 9111279
AN - SCOPUS:0030938883
SN - 0041-1132
VL - 37
SP - 411
EP - 417
JO - Transfusion
JF - Transfusion
IS - 4
ER -