Complete genome sequence of Paenibacillus strain Y4.12MC10, a novel Paenibacillus lautus strain isolated from obsidian hot spring in yellowstone national park

David A. Mead, Susan Lucas, Alex Copeland, Alla Lapidus, Jan Feng Cheng, David C. Bruce, Lynne A. Goodwin, Sam Pitluck, Olga Chertkov, Xiaojing Zhang, John C. Detter, Cliff S. Han, Roxanne Tapia, Miriam Land, Loren J. Hauser, Yun juan Chang, Nikos C. Kyrpides, Natalia N. Ivanova, Galina Ovchinnikova, Tanja WoykeCatherine Brumm, Rebecca Hochstein, Thomas Schoenfeld, Phillip Brumm

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Paenibacillus sp.Y412MC10 was one of a number of organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. The isolate was initially classified as a Geobacillus sp. Y412MC10 based on its isolation conditions and similarity to other organisms isolated from hot springs at Yellowstone National Park. Comparison of 16 S rRNA sequences within the Bacillales indicated that Geobacillus sp.Y412MC10 clustered with Paenibacillus species, and the organism was most closely related to Paenibacillus lautus. Lucigen Corp. prepared genomic DNA and the genome was se-quenced, assembled, and annotated by the DOE Joint Genome Institute. The genome sequence was deposited at the NCBI in October 2009 (NC_013406). The genome of Paenibacillus sp. Y412MC10 consists of one circular chromosome of 7,121,665 bp with an average G+C content of 51.2%. Comparison to other Paenibacillus species shows the organism lacks nitrogen fixation, antibiotic production and social interaction genes reported in other paenibacilli. The Y412MC10 genome shows a high level of synteny and homology to the draft sequence of Paenibacillus sp. HGF5, an organism from the Human Microbiome Project (HMP) Reference Genomes. This, combined with genomic CAZyme analysis, suggests an intestinal, rather than environmental origin for Y412MC10.

Original languageEnglish (US)
Pages (from-to)366-385
Number of pages20
JournalStandards in Genomic Sciences
Volume6
Issue number3
DOIs
StatePublished - 2012

Bibliographical note

Funding Information:
AC52-07NA27344, and Los Alamos National Laboratory under contract No. DE-AC02-06NA25396. This work was funded in part by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494).

Funding Information:
This work was performed under the auspices of the US Department of Energy Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract No. DE-

Keywords

  • Geobacillus sp. Y412MC10
  • Obsidian hot spring
  • Paenibacillus sp. Y412MC10

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