Complete genome sequence and lytic phase transcription profile of a Coccolithovirus

William H. Wilson; Declan C. Schroeder; Michael J. Allen; Matthew T.G. Holden; Julian Parkhill; Bart G. Barrell; Carol Churcher; Nancy Hamlin; Karen Mungall; Halina Norbertczak; Michael A. Quail; Claire Price; Ester Rabbinowitsch; Danielle Walker; Marie Craigon; Douglas Roy; Peter Ghazal

doi:10.1126/science.1113109

Complete genome sequence and lytic phase transcription profile of a Coccolithovirus

William H. Wilson, Declan C. Schroeder, Michael J. Allen, Matthew T.G. Holden, Julian Parkhill, Bart G. Barrell, Carol Churcher, Nancy Hamlin, Karen Mungall, Halina Norbertczak, Michael A. Quail, Claire Price, Ester Rabbinowitsch, Danielle Walker, Marie Craigon, Douglas Roy, Peter Ghazal

Research output: Contribution to journal › Article › peer-review

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Abstract

The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.

Original language	English (US)
Pages (from-to)	1090-1092
Number of pages	3
Journal	Science
Volume	309
Issue number	5737
DOIs	https://doi.org/10.1126/science.1113109
State	Published - Aug 12 2005
Externally published	Yes

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Wilson, W. H., Schroeder, D. C., Allen, M. J., Holden, M. T. G., Parkhill, J., Barrell, B. G., Churcher, C., Hamlin, N., Mungall, K., Norbertczak, H., Quail, M. A., Price, C., Rabbinowitsch, E., Walker, D., Craigon, M., Roy, D., & Ghazal, P. (2005). Complete genome sequence and lytic phase transcription profile of a Coccolithovirus. Science, 309(5737), 1090-1092. https://doi.org/10.1126/science.1113109

Wilson, WH, Schroeder, DC, Allen, MJ, Holden, MTG, Parkhill, J, Barrell, BG, Churcher, C, Hamlin, N, Mungall, K, Norbertczak, H, Quail, MA, Price, C, Rabbinowitsch, E, Walker, D, Craigon, M, Roy, D & Ghazal, P 2005, 'Complete genome sequence and lytic phase transcription profile of a Coccolithovirus', Science, vol. 309, no. 5737, pp. 1090-1092. https://doi.org/10.1126/science.1113109

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abstract = "The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.",

author = "Wilson, {William H.} and Schroeder, {Declan C.} and Allen, {Michael J.} and Holden, {Matthew T.G.} and Julian Parkhill and Barrell, {Bart G.} and Carol Churcher and Nancy Hamlin and Karen Mungall and Halina Norbertczak and Quail, {Michael A.} and Claire Price and Ester Rabbinowitsch and Danielle Walker and Marie Craigon and Douglas Roy and Peter Ghazal",

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doi = "10.1126/science.1113109",

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AU - Wilson, William H.

AU - Schroeder, Declan C.

AU - Allen, Michael J.

AU - Holden, Matthew T.G.

AU - Parkhill, Julian

AU - Barrell, Bart G.

AU - Churcher, Carol

AU - Hamlin, Nancy

AU - Mungall, Karen

AU - Norbertczak, Halina

AU - Quail, Michael A.

AU - Price, Claire

AU - Rabbinowitsch, Ester

AU - Walker, Danielle

AU - Craigon, Marie

AU - Roy, Douglas

AU - Ghazal, Peter

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AB - The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.

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Complete genome sequence and lytic phase transcription profile of a Coccolithovirus

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