Complete Amino Acid Sequence of a Papain-Solubilized Human Histocompatibility Antigen, HLA-B7. 2. Sequence Determination and Search for Homologies

Harry T. Orr, JoŚe A.López de Castro, Doron Lancet, Jack L. Strominger

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

The complete amino acid sequence of the pa-pain-solubilized heavy chain of a human histocompatibility antigen (HLA-B7) has been elucidated. It consists of a polypeptide of 271 residues (31 333 daltons). A single glycan moiety is attached to an asparagine residue at position 86 by an N-glycosidic bond. Two intrachain disulfide bonds, arranged linearly, involve half-cystine residues at positions 101 and 164 and at positions 203 and 259. They form two loops of 62 and 55 residues, respectively, separated by 38 residues. Computer analysis of the sequence suggests the existence of internal homology between the amino-terminal portion (residues 1-90) and the region of the first disulfide loop (residues 91-180). There is a significant homology between the second disulfide loop region of the chain (residues 182-271) and immunoglobulin (Ig) constant domains and '2-microglobulin [Orr, H. T., Lancet, D., Robb, R. J., López de Castro, J. A., & Strominger, J. L. (1979a) Nature (London) (in press)]. However, no such homology to Ig is apparent in the amino-terminal or in the first disulfide loop regions.

Original languageEnglish (US)
Pages (from-to)5711-5720
Number of pages10
JournalBiochemistry
Volume18
Issue number25
DOIs
StatePublished - Jan 1 1979

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HLA-B7 Antigen
Histocompatibility Antigens
Papain
Sequence Alignment
Disulfides
Amino Acid Sequence
Amino Acids
Immunoglobulins
Asparagine
Polysaccharides
Cysteine
Sequence Analysis
Pain
Peptides

Cite this

Complete Amino Acid Sequence of a Papain-Solubilized Human Histocompatibility Antigen, HLA-B7. 2. Sequence Determination and Search for Homologies. / Orr, Harry T.; Castro, JoŚe A.López de; Lancet, Doron; Strominger, Jack L.

In: Biochemistry, Vol. 18, No. 25, 01.01.1979, p. 5711-5720.

Research output: Contribution to journalArticle

Orr, Harry T. ; Castro, JoŚe A.López de ; Lancet, Doron ; Strominger, Jack L. / Complete Amino Acid Sequence of a Papain-Solubilized Human Histocompatibility Antigen, HLA-B7. 2. Sequence Determination and Search for Homologies. In: Biochemistry. 1979 ; Vol. 18, No. 25. pp. 5711-5720.
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