Complementary DNA cloning and expression of Pit-1/GHF-1 from the domestic turkey

E. A. Wong, J. L. Silsby, M. E. El Halawani

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41 Scopus citations

Abstract

The transition from egg laying to incubation activity in birds is associated with a dramatic rise in serum prolactin levels. To further our understanding of the regulation of prolactin gene expression in birds, a cDNA clone encoding turkey Pit-1/GHF-1 was isolated. The turkey cDNA, designated tPit-1/GHF-1, was 1,123 nucleotides in length and encoded a protein of 327 amino acids, including a conserved 80-amino-acid POU-specific domain and a 60-amino-acid POU homeodomain. tPit-1/GHF-1 POU-specific domain and POU- homeodomain showed 94-95% amino acid identity with the corresponding rat Pit- 1/GHF-1 domains. At its amino terminus, tPit-1/GHF-1 contained a 26-amino- acid insertion comparable to that found in the rat variant isoform, Pit-1β. Two other insertions of 38 and 7 amino acids were present and were not found in the mammalian protein. Levels of tPit-1/GHF-1 mRNA in pituitary tissue were examined at different phases of the turkey reproductive cycle by Northern blotting. tPit-1/GHF-1 mRNA was expressed as a 3.5-kb transcript, whose abundance remained relatively constant throughout the reproductive cycle. Thus, the dramatic rise in prolactin mRNA, observed during hyperprolactinemia in incubating turkey hens, was not associated with a concomitant increase in tPit-1/GHF-1 gene expression.

Original languageEnglish (US)
Pages (from-to)651-660
Number of pages10
JournalDNA and Cell Biology
Volume11
Issue number9
StatePublished - 1992

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