Competition between Coiled-Coil Structures and the Impact on Myosin-10 Bundle Selection

Kevin C. Vavra, Youlin Xia, Ronald S. Rock

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Coiled-coil fusions are a useful approach to enforce dimerization in protein engineering. However, the final structures of coiled-coil fusion proteins have received relatively little attention. Here, we determine the structural outcome of adjacent parallel and antiparallel coiled coils. The targets are coiled coils that stabilize myosin-10 in single-molecule biophysical studies. We reveal the solution structure of a short, antiparallel, myosin-10 coiled-coil fused to the parallel GCN4-p1 coiled coil. Surprisingly, this structure is a continuous, antiparallel coiled coil where GCN4-p1 pairs with myosin-10 rather than itself. We also show that longer myosin-10 segments in these parallel/antiparallel fusions are dynamic and do not fold cooperatively. Our data resolve conflicting results on myosin-10 selection of actin filament bundles, demonstrating the importance of understanding coiled-coil orientation and stability.

Original languageEnglish (US)
Pages (from-to)2517-2527
Number of pages11
JournalBiophysical journal
Issue number11
StatePublished - Jun 7 2016

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Publisher Copyright:
© 2016 Biophysical Society.


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