Comparison of three serological assays to determine the cross-reactivity of antibodies from eight genetically diverse U.S. Swine influenza viruses

Brad Leuwerke, Pravina Kitikoon, Richard Evans, Eileen Thacker

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Swine influenza virus is an economically important pathogen to the U.S. swine industry. New influenza subtypes and isolates within subtypes with different genetic and antigenic makeup have recently emerged in U.S. swineherds. As a result of the emergence of these new viruses, diagnosticians' ability to accurately diagnose influenza infection in pigs and develop appropriate vaccine strategies has become increasingly difficult. The current study compares the ability of subtype-specific commercial enzyme-linked immunosorbent assays (ELISA), hemagglutination inhibition (HI), and serum neutralization (SN) assays to detect antibodies elicited by multiple isolates within different subtypes of influenza virus. Pigs were infected with genetically and antigenically different isolates of the 3 major circulating subtypes within populations of swine (H1N1, H1N2, and H3N2). Serum was collected when all pigs within a group collectively reached HI reciprocal titers ≥ 160 against that group's homologous challenge virus. The antibody cross-reactivity of the sera between isolates was determined using ELISA, HI, and SN assays. In addition, the correlation between the 3 assays was determined. The assays differed in their ability to detect antibodies produced by the viruses used in the study. The results provide important information to diagnostic laboratories, veterinarians, and swine producers on the ability of 3 common serological assays used in identifying infection with influenza in pigs.

Original languageEnglish (US)
Pages (from-to)426-432
Number of pages7
JournalJournal of Veterinary Diagnostic Investigation
Volume20
Issue number4
DOIs
StatePublished - Jul 1 2008
Externally publishedYes

Bibliographical note

Funding Information:
The authors would like to thank Barbara Erickson, Nancy Upchurch, and the students of the Thacker Laboratory for their assistance in this project. The project was funded by the National Pork Board.

Keywords

  • Enzyme-linked immunosorbent assay
  • Hemagglutination inhibition assay
  • Pigs
  • Serum neutralizing assay
  • Swine influenza viruses

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