Comparison of three lipid formulations for synthetic surfactant with a surfactant protein B analog

Frans J. Walther, José M. Hernández-Juviel, Larry M. Gordon, Alan J. Waring, Patrick Stenger, Joseph A. Zasadzinski

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Surfactant protein B (SP-B) is an essential component of pulmonary surfactant. Synthetic dimeric SP-B1-25 (SP-B1-25), a peptide based on the N-terminal domain of human SP-B, efficiently mimics the functional properties of SP-B. The authors investigated the optimum lipid composition for SP-B1-25 by comparing the effects of natural lung lavage lipids (NLL), a synthetic equivalent of NLL (synthetic lavage lipids SLL), and a standard lipid mixture (TL) on the activities of SP-B 1-25. Surfactant preparations were formulated by mixing 2 mol% SP-B1-25 in NNL, SLL, and TL. Calfactant, a calf lung lavage extract with SP-B and SP-C, was a positive control and lipids without peptide were negative controls. Minimum surface tension measured on a captive bubble surfactometer was similar for the three SP-B1-25 surfactant preparations and calfactant. The effects on lung function were compared in ventilated, lavaged, surfactant-deficient rats. Oxygenation and lung volumes were consistently higher in rats treated with calfactant and SP-B1-25 in NLL or SLL than in rats treated with SP-B1-25 in TL. Fourier transform infrared spectra observed abnormal secondary conformations for SP-B1-25 in TL as a possible cause for the reduced lung function. Lipid composition plays a crucial role in the in vitro and in vivo functions of SP-B1-25 in surfactant preparations.

Original languageEnglish (US)
Pages (from-to)563-579
Number of pages17
JournalExperimental Lung Research
Volume31
Issue number6
DOIs
StatePublished - Jul 1 2005

Keywords

  • Captive bubble surfactometry
  • Dimeric SP-B peptide
  • Fourier transform infrared spectroscopy
  • Lung function
  • Rats

Fingerprint Dive into the research topics of 'Comparison of three lipid formulations for synthetic surfactant with a surfactant protein B analog'. Together they form a unique fingerprint.

  • Cite this