Comparison of the level of mRNA encoding m1 and m2 muscarinic receptors in brains of young and aged rats

Shou Zhen Wang; Sheng Zu Zhu; James A. Joseph; Esam E El-Fakahany

doi:10.1016/0304-3940(92)90008-U

Comparison of the level of mRNA encoding m1 and m2 muscarinic receptors in brains of young and aged rats

Shou Zhen Wang
, Sheng Zu Zhu
, James A. Joseph
, Esam E El-Fakahany

Experimental and Clinical Pharmacology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We compared the concentration of mRNA encoding the m1 and m2 muscarinic receptors in several brain regions obtained from young (5-8 months) and aged (24-28 months) male Fischer 344 rats. DNA-excess solution hybridization was employed as a quantitative measure of mRNA concentration. The results indicate the absence of changes in the m1 receptor message with aging in the cerebral cortex, hippocampus and striatum. While there was no statistically significant aging-associated alteration in the concentration of the message encoding the m2 receptor in the thalamus, midbrain, cerebellum and brainstem, there was a decrease in the message level in the hypothalamus.

Original language	English (US)
Pages (from-to)	149-152
Number of pages	4
Journal	Neuroscience Letters
Volume	145
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(92)90008-U
State	Published - Oct 12 1992

Bibliographical note

Funding Information:
This work was supported in part by grants from the U.S. Army Research Office and the National Institutes of Health. The authors would like to thank Susan Rasmussen and Colette Lunday for their secretarial assistance.

Keywords

Aging
Muscarinic receptor
Rat brain
Receptor subtype
Solution hybridization
mRNA

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abstract = "We compared the concentration of mRNA encoding the m1 and m2 muscarinic receptors in several brain regions obtained from young (5-8 months) and aged (24-28 months) male Fischer 344 rats. DNA-excess solution hybridization was employed as a quantitative measure of mRNA concentration. The results indicate the absence of changes in the m1 receptor message with aging in the cerebral cortex, hippocampus and striatum. While there was no statistically significant aging-associated alteration in the concentration of the message encoding the m2 receptor in the thalamus, midbrain, cerebellum and brainstem, there was a decrease in the message level in the hypothalamus.",

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AU - Zhu, Sheng Zu

AU - Joseph, James A.

AU - El-Fakahany, Esam E

N1 - Funding Information: This work was supported in part by grants from the U.S. Army Research Office and the National Institutes of Health. The authors would like to thank Susan Rasmussen and Colette Lunday for their secretarial assistance.

PY - 1992/10/12

Y1 - 1992/10/12

N2 - We compared the concentration of mRNA encoding the m1 and m2 muscarinic receptors in several brain regions obtained from young (5-8 months) and aged (24-28 months) male Fischer 344 rats. DNA-excess solution hybridization was employed as a quantitative measure of mRNA concentration. The results indicate the absence of changes in the m1 receptor message with aging in the cerebral cortex, hippocampus and striatum. While there was no statistically significant aging-associated alteration in the concentration of the message encoding the m2 receptor in the thalamus, midbrain, cerebellum and brainstem, there was a decrease in the message level in the hypothalamus.

AB - We compared the concentration of mRNA encoding the m1 and m2 muscarinic receptors in several brain regions obtained from young (5-8 months) and aged (24-28 months) male Fischer 344 rats. DNA-excess solution hybridization was employed as a quantitative measure of mRNA concentration. The results indicate the absence of changes in the m1 receptor message with aging in the cerebral cortex, hippocampus and striatum. While there was no statistically significant aging-associated alteration in the concentration of the message encoding the m2 receptor in the thalamus, midbrain, cerebellum and brainstem, there was a decrease in the message level in the hypothalamus.

KW - Aging

KW - Muscarinic receptor

KW - Rat brain

KW - Receptor subtype

KW - Solution hybridization

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Comparison of the level of mRNA encoding m1 and m2 muscarinic receptors in brains of young and aged rats

Abstract

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