Incorporation of3H-Thymidine into DNA-synthesizing cells of the gastrointestinal tract of the rat was examined following administration of the isotope by intraperitoneal and intravenous routes. Estimates of whole tissue incorporation expressed as DPM/mg dry weight and of proliferating cells expressed as DPM/crypt or gland in the different segments of the gut indicated no differences in the degree of3H-TdR uptake into DNA following intraperitoneal or intravenous routes of administration. The possibility of misdirected I.P. injections was examined following injection of3H-TdR into the cecum or bladder. DPM/mg wet weight of gastrointestinal tissues indicated reduction in the uptake of3H-TdR into DNA of intestinal tissues following intracecal and intrabladder administration of3H-Tdr. The intraperitoneal route of administration of3H-TdR appears to be equally effective in the distribution of the isotope into different segments of the gut when compared to the intravenous route and is a more convenient method in studies of cell production in the gastrointestinal tract of the rat.
|Original language||English (US)|
|Number of pages||8|
|Journal||Virchows Archiv B Cell Pathology Including Molecular Pathology|
|State||Published - Jan 1 1985|
- Gastrointestinal tract
- Routes of delivery
- Tritiated thymidine