Comparison of the Effects of N-Ethylmaleimide and lodoacetamide on Red Blood Cell Membranes

David W Allen, Shirley Cadman

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Although both NEM and IA completely inhibited G3PD activity and decreased RBC ATP levels 40%, IA produced little effect on RBC filterability whereas NEM greatly reduced the filtration rate. IA caused only an isolated increase of band 6 (G3PD) and no change in membrane protein content. However, the decreased filter-ability of NEM-treated RBC was associated with significantly increased membrane-associated nonhemoglobin protein which appeared as a complex array of polypeptide bands on PAGE SDS.

Original languageEnglish (US)
Pages (from-to)318-321
Number of pages4
JournalProceedings of the Society for Experimental Biology and Medicine
Volume152
Issue number3
DOIs
StatePublished - Jul 1976

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