Comparison of the antimicrobial activity of six irrigants on primary endodontic pathogens

The purpose of this study was to compare the antimicrobial activities of 6% and 3% sodium hypochlorite (NaOCl), 2% and 0.12% chlorhexidine gluconate (CHX), and 0.01% and 0.005% doxycycline (Doxy) on four microorganisms associated with primary endodontic infections. The agar diffusion test was used to measure antimicrobial activities of these agents against Peptostreptococcus micros, Prevotella intermedia, Streptococcus sanguis, and Lactobacillus acidophilus. Minimum inhibitory concentration analysis was performed using the macrodilution method. For three of the four microorganisms, the general order of antimicrobial effectiveness was 0.01% Doxy >0.005% Doxy >6% NaOCl >3% NaOCl >2% CHX >0.12% CHX. For L acidophilus, the order of effectiveness was 6% NaOCl >3% NaOCl >2% CHX >0.01 % Doxy >0.005% Doxy >0.12% CHX. The 6% NaOCl showed significantly greater zones of inhibition than 3% NaOCl for all endopathogens tested.