Aim: Cigarette smoking influences DNA methylation genome wide, in newborns from pregnancy exposure and in adults from personal smoking. Whether a unique methylation signature exists for in utero exposure in newborns is unknown. Materials & methods: We separately meta-analyzed newborn blood DNA methylation (assessed using Illumina450k Beadchip), in relation to sustained maternal smoking during pregnancy (9 cohorts, 5648 newborns, 897 exposed) and adult blood methylation and personal smoking (16 cohorts, 15907 participants, 2433 current smokers). Results & conclusion: Comparing meta-analyses, we identified numerous signatures specific to newborns along with many shared between newborns and adults. Unique smoking-associated genes in newborns were enriched in xenobiotic metabolism pathways. Our findings may provide insights into specific health impacts of prenatal exposure on offspring.
Bibliographical noteFunding Information:
29Department of Epidemiology & Prevention, Public Health Sciences, Wake Forest School of Medicine, Winston-Salem, NC 27101, USA 30Department of Environmental Medicine & Public Health, Icahn School of Medicine at Mount Sinai, New York City, NY 10029, USA 31Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, The Netherlands 32Department of Clinical Chemistry, Erasmus University Medical Center, Rotterdam, The Netherlands 33Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France 34Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden 35Westat, Durham, NC 27703, USA 36Department of Environmental Health Sciences, Columbia University Mailman School of Public Health, New York City, NY 10032, USA 37Departments of Obstetrics & Gynecology & Pathology, Duke University School of Medicine, Durham, NC 27708, USA 38Department of Pediatrics, Oslo University Hospital, Oslo, Norway 39National Institute of Public Health, Oslo, Norway 40Centre for Cognitive Ageing & Cognitive Epidemiology, Edinburgh, UK 41Department of Psychology, University of Edinburgh, Edinburgh, UK 42Division of Mental & Physical Health, Norwegian Institute of Public Health, Oslo, Norway 43Institute of Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany 44Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA 45MRC Epidemiology Unit, Institute of Metabolic Science, University of Cambridge, Cambridge, UK 46Department of Paediatrics, University of Cambridge, Cambridge, UK 47Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA 48Autonomous Metropolitan University Iztapalapa, Mexico City, Mexico 49Nutrition & Metabolism Section, International Agency for Research on Cancer, Lyon, France 50Center for Research on Population Health, National Institute of Public Health, Mexico 51Hubert Department of Global Health, Emory University, Atlanta, GA 30329, USA 52Institute of Molecular Medicine & Human Genetics Center, University of Texas Health Science Center at Houston, Houston, TX 77225, USA 53Population Sciences Branch, National Heart, Lung, & Blood Institute, National Institutes of Health, Bethesda, MD 01702, USA *Author for correspondence: email@example.com
Funded by the Intramural Research Program of National Institutes of Health (National Institute of Environmental Health Sciences ZO1 ES49019) in addition to funding for individual authors and cohorts listed in the Supplementary Acknowledgements and Funding document. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.
- cigarette smoking
- maternal exposure