Comparison of short- and long-term exposure effects of cruciferous and apiaceous vegetables on carcinogen metabolizing enzymes in Wistar rats

Jae Kyeom Kim, Noemia Strapazzon, Cynthia M. Gallaher, Dwight R. Stoll, William Thomas, Daniel D. Gallaher, Sabrina P. Trudo

Research output: Contribution to journalArticle

Abstract

Cruciferous and apiaceous vegetables may be chemopreventive due to their ability to modulate carcinogen-metabolizing enzymes but whether the effects on such enzymes are sustained over time is unknown. To examine the short- and long-term effects of the vegetables, rats were fed one of four diets for 7, 30, or 60 d: AIN-93G, CRU (21% cruciferous vegetables-fresh broccoli, green cabbage, watercress), API (9% apiaceous vegetables - fresh parsnips, celery), or API + CRU (10.5% CRU + 4.5% API). Although CRU increased activity and protein expression of cytochrome P450 (CYP) 1A1 and CYP1A2 after 7 d, only activity was sustained after 30 and 60 d. There was a trend towards an interaction between the length of feeding period and CRU for CYP1A1 activity; activity increased with greater time of feeding. API increased CYP1A2 activity but decreased sulfotransferase 1A1 activity after 7 d, although not at later times. Altogether, increased CYP1A activity by CRU was maintained with long term feeding while protein amount decreased, suggesting influence by mechanisms other than, or in addition to, transcriptional regulation. Thus, response patterns and interactions with length of feeding may differ, depending upon the types of vegetables and enzymes, requiring caution when interpreting the results of short-term feeding studies.

Original languageEnglish (US)
Pages (from-to)194-202
Number of pages9
JournalFood and Chemical Toxicology
Volume108
DOIs
StatePublished - Oct 1 2017

Fingerprint

vegetables
enzymes
Vegetables
Carcinogens
Wistar Rats
Enzymes
Specific Gravity
Medieval History
Application programming interfaces (API)
raw vegetables
carcinogens
rats
Cytochrome P-450 CYP1A2
Brassica
Proteins
Staphylococcal Pneumonia
parsnips
watercress
sulfotransferases
celery

Keywords

  • Apiaceous vegetable
  • Biotransformation enzyme
  • Cruciferous vegetable
  • Cytochrome P450
  • Phase II enzymes

ASJC Scopus subject areas

  • Food Science
  • Toxicology

MeSH PubMed subject areas

  • Journal Article

Cite this

Comparison of short- and long-term exposure effects of cruciferous and apiaceous vegetables on carcinogen metabolizing enzymes in Wistar rats. / Kim, Jae Kyeom; Strapazzon, Noemia; Gallaher, Cynthia M.; Stoll, Dwight R.; Thomas, William; Gallaher, Daniel D.; Trudo, Sabrina P.

In: Food and Chemical Toxicology, Vol. 108, 01.10.2017, p. 194-202.

Research output: Contribution to journalArticle

Kim, Jae Kyeom; Strapazzon, Noemia; Gallaher, Cynthia M.; Stoll, Dwight R.; Thomas, William; Gallaher, Daniel D.; Trudo, Sabrina P. / Comparison of short- and long-term exposure effects of cruciferous and apiaceous vegetables on carcinogen metabolizing enzymes in Wistar rats.

In: Food and Chemical Toxicology, Vol. 108, 01.10.2017, p. 194-202.

Research output: Contribution to journalArticle

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