Comparison of "polarization inversion with spin exchange at magic angle" and "geometric analysis of labeled alanines" methods for transmembrane helix alignment

Vitaly V. Vostrikov, Christopher V. Grant, Anna E. Daily, Stanley J. Opella, Roger E. Koeppe

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Using the model α-helical peptide acetyl-GGALW5LALALALALALALW19LAGA-ethanolamide ("GWALP23"), we have compared the polarization inversion with spin exchange at magic angle method and geometric analysis of labeled alanines method for estimating the transmembrane helix orientation. For GWALP23 in bilayers of a short lipid, dilauroylphosphatidylcholine, we find general agreement between the two methods, with a static helix tilt of about 11°-13° with respect to the bilayer normal.

Original languageEnglish (US)
Pages (from-to)12584-12585
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number38
DOIs
StatePublished - Sep 24 2008

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