Comparison of multiple HLA-A alleles at the DNA level by using southern blotting and HLA-A-specific probes

B. H. Koller, F. E. Ward, R. DeMars, H. T. Orr

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15 Citations (Scopus)

Abstract

Numerous alleles of the HLA-A gene have been serologically identified. In this report we present a rapid and straightforward means to assess HLA-A polymorphism at the genomic level. Using 5' and 3' HLA-A-specific DNA probes and Southern blotting, we have placed the recognition sequences for five endonucleases relative to the coding regions of 15 HLA-A alleles. These data permit two interesting conclusions: 1) four of the HLA-A alleles studied are associated with unique restriction fragments, and 2) HLA-A alleles of a cross-reactive group are more closely related at the DNA level than are noncross-reactive alleles.

Original languageEnglish (US)
Pages (from-to)4229-4234
Number of pages6
JournalJournal of Immunology
Volume135
Issue number6
StatePublished - Dec 1 1985

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HLA-A Antigens
Southern Blotting
Alleles
DNA
HLA DNA Probes
Endonucleases
Genes

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Comparison of multiple HLA-A alleles at the DNA level by using southern blotting and HLA-A-specific probes. / Koller, B. H.; Ward, F. E.; DeMars, R.; Orr, H. T.

In: Journal of Immunology, Vol. 135, No. 6, 01.12.1985, p. 4229-4234.

Research output: Contribution to journalArticle

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