TY - JOUR
T1 - Comparison of methods for differentiation of Salmonella enterica serovar Enteritidis phage type 4 isolates
AU - Lopes, Vanessa C.
AU - Velayudhan, Binu T.
AU - Halvorson, David A.
AU - Lauer, Dale C.
AU - Gast, Richard K.
AU - Nagaraja, Kakambi V.
PY - 2004/5
Y1 - 2004/5
N2 - Objective - To compare molecular typing methods for the differentiation of Salmonella enterica serovar Enteritidis phage type (PT) 4 isolates that allowed for the determination of their genetic relatedness. Sample Population - 27 Salmonella Enteriticis PT 4 strains isolated in the United States and Europe. Procedure - Several molecular typing methods were performed to assess their ability to genetically differentiate among Salmonella Enteritidis PT 4 isolates. Results of pulse-field gel electrophoresis (PFGE), repetitive polymerase chain reaction (PCR) assay, 16S rRNA gene sequencing, random amplification of polymorphic DNA (RAPD), PCR-restriction fragment length polymorphism of 16S rRNA, and antimicrobial susceptibility were evaluated. Results - Compared with results for other techniques, results for the RAPD typing method with the RAPD1 primer reveal that it was the most discriminatory fingerprinting technique, and it allowed us to cluster Salmonella Enteritidis PT 4 isolates on the basis of their genetic similarity. Conclusions and Clinical Relevance - This study revealed the value of RAPD with the RAPD1 primer as a tool for epidemiologic investigations of Salmonella Enteritidis PT 4. It can be used in conjunction with PFGE and phage typing to determine the genetic relatedness of Salmonella Enteritidis isolates involved in outbreaks of disease. A reliable and highly discriminatory method for epidemiologic investigations is critical to allow investigators to identify the source of infections and consequently prevent the spread of Salmonella Enteritidis PT 4.
AB - Objective - To compare molecular typing methods for the differentiation of Salmonella enterica serovar Enteritidis phage type (PT) 4 isolates that allowed for the determination of their genetic relatedness. Sample Population - 27 Salmonella Enteriticis PT 4 strains isolated in the United States and Europe. Procedure - Several molecular typing methods were performed to assess their ability to genetically differentiate among Salmonella Enteritidis PT 4 isolates. Results of pulse-field gel electrophoresis (PFGE), repetitive polymerase chain reaction (PCR) assay, 16S rRNA gene sequencing, random amplification of polymorphic DNA (RAPD), PCR-restriction fragment length polymorphism of 16S rRNA, and antimicrobial susceptibility were evaluated. Results - Compared with results for other techniques, results for the RAPD typing method with the RAPD1 primer reveal that it was the most discriminatory fingerprinting technique, and it allowed us to cluster Salmonella Enteritidis PT 4 isolates on the basis of their genetic similarity. Conclusions and Clinical Relevance - This study revealed the value of RAPD with the RAPD1 primer as a tool for epidemiologic investigations of Salmonella Enteritidis PT 4. It can be used in conjunction with PFGE and phage typing to determine the genetic relatedness of Salmonella Enteritidis isolates involved in outbreaks of disease. A reliable and highly discriminatory method for epidemiologic investigations is critical to allow investigators to identify the source of infections and consequently prevent the spread of Salmonella Enteritidis PT 4.
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U2 - 10.2460/ajvr.2004.65.538
DO - 10.2460/ajvr.2004.65.538
M3 - Article
C2 - 15141870
AN - SCOPUS:3042664039
SN - 0002-9645
VL - 65
SP - 538
EP - 543
JO - American journal of veterinary research
JF - American journal of veterinary research
IS - 5
ER -