Comparison of kinetic models to describe high pressure and gamma irradiation used to inactivate Vibrio vulnificus and Vibrio parahaemolyticus prepared in buffer solution and in whole oysters

Xiaopei Hu, Parameswarakumar Mallikarjunan, Jaheon Koo, Linda S. Andrews, Michael L. Jahncke

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Abstract

Comparisons of different models in inactivation kinetics were conducted on data obtained from high-pressure and gamma-irradiation processing. Vibrio vulnificus (MO-624) and Vibrio parahaemolyticus (O3:K6 TX-2103) suspended in phosphate-buffered saline (pH 7.4, 10 7 CFU/ml) were exposed to pressures from 207 to 379 MPa for 1 to 20 min. Inoculated whole oysters (10 6 CFU/g) were exposed to pressure from 276 to 379 MPa for 1 to 15 min. Pure cultures and inoculated oysters (10 6 CFU/g) also were irradiated (gamma irradiation) at doses of less than 3 kGy. Four mathematical models, the Bigelow model, Arrhenius equation, Fermi equation, and Weibull frequency distributions, were applied to microbial survival data, and performances of the different kinetic models were compared. Weibull frequency distributions can predict the high-pressure inactivation of Vibrio spp. with more accuracy in both pure cultures and inoculated oyster samples. The Fermi model provided a better description of gamma-irradiation inactivation kinetics compared with the traditional Bigelow model.

Original languageEnglish (US)
Pages (from-to)292-295
Number of pages4
JournalJournal of food protection
Volume68
Issue number2
DOIs
StatePublished - Jan 1 2005

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Vibrio vulnificus
Vibrio parahaemolyticus
Ostreidae
oysters
gamma radiation
Buffers
buffers
kinetics
Pressure
inactivation
Vibrio
Theoretical Models
Phosphates
mathematical models
phosphates
dosage

Cite this

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title = "Comparison of kinetic models to describe high pressure and gamma irradiation used to inactivate Vibrio vulnificus and Vibrio parahaemolyticus prepared in buffer solution and in whole oysters",
abstract = "Comparisons of different models in inactivation kinetics were conducted on data obtained from high-pressure and gamma-irradiation processing. Vibrio vulnificus (MO-624) and Vibrio parahaemolyticus (O3:K6 TX-2103) suspended in phosphate-buffered saline (pH 7.4, 10 7 CFU/ml) were exposed to pressures from 207 to 379 MPa for 1 to 20 min. Inoculated whole oysters (10 6 CFU/g) were exposed to pressure from 276 to 379 MPa for 1 to 15 min. Pure cultures and inoculated oysters (10 6 CFU/g) also were irradiated (gamma irradiation) at doses of less than 3 kGy. Four mathematical models, the Bigelow model, Arrhenius equation, Fermi equation, and Weibull frequency distributions, were applied to microbial survival data, and performances of the different kinetic models were compared. Weibull frequency distributions can predict the high-pressure inactivation of Vibrio spp. with more accuracy in both pure cultures and inoculated oyster samples. The Fermi model provided a better description of gamma-irradiation inactivation kinetics compared with the traditional Bigelow model.",
author = "Xiaopei Hu and Parameswarakumar Mallikarjunan and Jaheon Koo and Andrews, {Linda S.} and Jahncke, {Michael L.}",
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AU - Hu, Xiaopei

AU - Mallikarjunan, Parameswarakumar

AU - Koo, Jaheon

AU - Andrews, Linda S.

AU - Jahncke, Michael L.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Comparisons of different models in inactivation kinetics were conducted on data obtained from high-pressure and gamma-irradiation processing. Vibrio vulnificus (MO-624) and Vibrio parahaemolyticus (O3:K6 TX-2103) suspended in phosphate-buffered saline (pH 7.4, 10 7 CFU/ml) were exposed to pressures from 207 to 379 MPa for 1 to 20 min. Inoculated whole oysters (10 6 CFU/g) were exposed to pressure from 276 to 379 MPa for 1 to 15 min. Pure cultures and inoculated oysters (10 6 CFU/g) also were irradiated (gamma irradiation) at doses of less than 3 kGy. Four mathematical models, the Bigelow model, Arrhenius equation, Fermi equation, and Weibull frequency distributions, were applied to microbial survival data, and performances of the different kinetic models were compared. Weibull frequency distributions can predict the high-pressure inactivation of Vibrio spp. with more accuracy in both pure cultures and inoculated oyster samples. The Fermi model provided a better description of gamma-irradiation inactivation kinetics compared with the traditional Bigelow model.

AB - Comparisons of different models in inactivation kinetics were conducted on data obtained from high-pressure and gamma-irradiation processing. Vibrio vulnificus (MO-624) and Vibrio parahaemolyticus (O3:K6 TX-2103) suspended in phosphate-buffered saline (pH 7.4, 10 7 CFU/ml) were exposed to pressures from 207 to 379 MPa for 1 to 20 min. Inoculated whole oysters (10 6 CFU/g) were exposed to pressure from 276 to 379 MPa for 1 to 15 min. Pure cultures and inoculated oysters (10 6 CFU/g) also were irradiated (gamma irradiation) at doses of less than 3 kGy. Four mathematical models, the Bigelow model, Arrhenius equation, Fermi equation, and Weibull frequency distributions, were applied to microbial survival data, and performances of the different kinetic models were compared. Weibull frequency distributions can predict the high-pressure inactivation of Vibrio spp. with more accuracy in both pure cultures and inoculated oyster samples. The Fermi model provided a better description of gamma-irradiation inactivation kinetics compared with the traditional Bigelow model.

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