Salmonella Typhimurium DT104 is a zoonotic enteropathogen of increasing concern for human health. In this study, the influence of growth phase on invasiveness of a S. Typhimurium DT104 field isolate and two reference strains (SL1344 and ATCC 14028) was compared in IPEC J2 cells and mucosal explants from porcine ileum. Internalized bacteria were quantified by a gentamicin resistance assay. After 90 min of exposure to the apical aspect of epithelial monolayers or luminal surface of explants, internalization of all S. Typhimurium strains in mid-logarithmic phase of bacterial growth was comparable. Internalization of stationary phase bacteria was reduced relative to log phase bacteria, with DT104 exhibiting the greatest decrease. Growth phase-related differences in S. Typhimurium invasion are similar in porcine intestinal epithelial cells and mucosal explants, but may be greater in multidrug-resistant strains.
|Original language||English (US)|
|Number of pages||7|
|Journal||Comparative Immunology, Microbiology and Infectious Diseases|
|State||Published - Jan 2008|
Bibliographical noteFunding Information:
This work was supported in part by National Institutes of Health Grant R01 DA-10200.
- Bacterial strain
- Growth phase
- Intestinal epithelium
- Multidrug resistance