Comparison of growth phase on Salmonella enterica serovar Typhimurium invasion in an epithelial cell line (IPEC J2) and mucosal explants from porcine small intestine

Lisa D. Schmidt, Laura J. Kohrt, David R. Brown

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20 Scopus citations

Abstract

Salmonella Typhimurium DT104 is a zoonotic enteropathogen of increasing concern for human health. In this study, the influence of growth phase on invasiveness of a S. Typhimurium DT104 field isolate and two reference strains (SL1344 and ATCC 14028) was compared in IPEC J2 cells and mucosal explants from porcine ileum. Internalized bacteria were quantified by a gentamicin resistance assay. After 90 min of exposure to the apical aspect of epithelial monolayers or luminal surface of explants, internalization of all S. Typhimurium strains in mid-logarithmic phase of bacterial growth was comparable. Internalization of stationary phase bacteria was reduced relative to log phase bacteria, with DT104 exhibiting the greatest decrease. Growth phase-related differences in S. Typhimurium invasion are similar in porcine intestinal epithelial cells and mucosal explants, but may be greater in multidrug-resistant strains.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume31
Issue number1
DOIs
StatePublished - Jan 2008

Bibliographical note

Funding Information:
This work was supported in part by National Institutes of Health Grant R01 DA-10200.

Keywords

  • Bacterial strain
  • Growth phase
  • Intestinal epithelium
  • Multidrug resistance

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