Abstract
Salmonella Typhimurium DT104 is a zoonotic enteropathogen of increasing concern for human health. In this study, the influence of growth phase on invasiveness of a S. Typhimurium DT104 field isolate and two reference strains (SL1344 and ATCC 14028) was compared in IPEC J2 cells and mucosal explants from porcine ileum. Internalized bacteria were quantified by a gentamicin resistance assay. After 90 min of exposure to the apical aspect of epithelial monolayers or luminal surface of explants, internalization of all S. Typhimurium strains in mid-logarithmic phase of bacterial growth was comparable. Internalization of stationary phase bacteria was reduced relative to log phase bacteria, with DT104 exhibiting the greatest decrease. Growth phase-related differences in S. Typhimurium invasion are similar in porcine intestinal epithelial cells and mucosal explants, but may be greater in multidrug-resistant strains.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 63-69 |
| Number of pages | 7 |
| Journal | Comparative Immunology, Microbiology and Infectious Diseases |
| Volume | 31 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2008 |
Bibliographical note
Funding Information:This work was supported in part by National Institutes of Health Grant R01 DA-10200.
Keywords
- Bacterial strain
- Growth phase
- Intestinal epithelium
- Multidrug resistance