Several clones of nonproducing cells were isolated from a continuous culture of hybridoma cells, which were originally producing antibody. Their behavior was compared to that of the producing cells in batch culture. The growth kinetics of five out of six clones exhibited higher specific growth rate, higher yield of cell mass on glutamine, and lower yields of lactate and ammonium. The implications of the comparisons for growth of hybridoma cultures are discussed.
Bibliographical noteFunding Information:
This research was supported by grants from the National Science Foundation (ECE-8512427, ECE-8552670) and Ecolab, Inc., St. Paul, MN. K.K.F. was the recipient of a fellowship from the Amoco Foundation. We thank Dr, Robert Vessella for providing us with the AFP-27 cell line and for useful discussions.
- batch culture
- growth kinetics
- nonproducing cells
- producing cells