Comparison of growth kinetics of producing and nonproducing hybridoma cells in batch culture

Kelly K. Frame, Wei Shou Hu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Several clones of nonproducing cells were isolated from a continuous culture of hybridoma cells, which were originally producing antibody. Their behavior was compared to that of the producing cells in batch culture. The growth kinetics of five out of six clones exhibited higher specific growth rate, higher yield of cell mass on glutamine, and lower yields of lactate and ammonium. The implications of the comparisons for growth of hybridoma cultures are discussed.

Original languageEnglish (US)
Pages (from-to)690-696
Number of pages7
JournalEnzyme and Microbial Technology
Volume13
Issue number9
DOIs
StatePublished - Sep 1991

Keywords

  • Hybridoma
  • batch culture
  • growth kinetics
  • nonproducing cells
  • producing cells

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