Comparison of core-shell particles and sub-2μm fully porous particles for use as ultrafast second dimension columns in two-dimensional liquid chtomatography

Imad A. Haidar Ahmad, Arianne Soliven, Robert C. Allen, Marcelo Filgueira, Peter W. Carr

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The peak capacity of small columns packed with 2.7. μm core-shell particles and 1.8. μm fully porous particles were compared at high temperatures using very steep (fast) gradient conditions and quite high linear velocities using the same instrument configuration as used to transfer first dimension effluent to the second dimension column as done in on-line comprehensive two-dimensional liquid chromatography. The experimental peak capacities of small columns (2.1. mm. ×. 30. mm) packed with both types of particles were measured with fast gradients (9. s to 2. min) at high temperature (95. °C) using both the same flow rate (1.75. mL/min) and then at different flow rates at the same pressure (400. bar). Equal or slightly better peak capacities were achieved with the core-shell particle columns as compared to the fully porous particle columns at the same backpressure or the same flow rate. However, core-shell particles offer a real advantage over the smaller, fully porous particles because they can be operated at higher flow rates thus gradient mixer flush out and column reequilibration can be done in less time thereby allowing a greater fraction of the second dimension cycle time to be dedicated to the gradient time.

Original languageEnglish (US)
Pages (from-to)31-38
Number of pages8
JournalJournal of Chromatography A
Volume1386
DOIs
StatePublished - Mar 20 2015

Keywords

  • Core-shell particles
  • High temperature liquid chromatography
  • Liquid chromatography
  • Sub 2μm particles
  • Two dimensional liquid chromatography
  • Ultra fast liquid chromatography

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