Comparison of bile acid synthesis determined by isotope dilution versus fecal acidic sterol output in human subjects

William C Duane, David E. Holloway, Scot W. Hutton, Patricia J. Corcoran, Nancy A. Haas

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Fecal acidic sterol output has been found to be much lower than bile acid synthesis determined by isotope dilution (J. Lipid Res. 17: 77, 1976). Because of this confusing discrepancy, we compared these 2 measurements done simultaneously on 13 occasions in 5 normal volunteers. In contrast to previous findings, bile acid synthesis by the Lindstedt isotope dilution method averaged 16.3% lower than synthesis simultaneously determined by fecal acidic sterol output (95% confidence limit for the difference -22.2 to -10.4%). When one-sample determinations of bile acid pools were substituted for Lindstedt pools, bile acid synthesis by isotope dilution averaged 5.6% higher than synthesis by fecal acidic sterol output (95% confidence limits -4.9 to 16.1%). These data indicate that the 2 methods yield values in reasonably close agreement with one another. If anything, fecal acidic sterol outputs are slightly higher than synthesis by isotope dilution.

Original languageEnglish (US)
Pages (from-to)345-348
Number of pages4
JournalLipids
Volume17
Issue number5
DOIs
StatePublished - May 1 1982

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