Comparative transcriptomics of steinernema and caenorhabditis single embryos reveals orthologous gene expression convergence during late embryogenesis

Marissa Macchietto, Dristi Angdembey, Negar Heidarpour, Lorrayne Serra, Bryan Rodriguez, Nicole El-Ali, Ali Mortazavi

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Cells express distinct sets of genes in a precise spatio-temporal manner during embryonic development. There is a wealth of information on the deterministic embryonic development of Caenorhabditis elegans, but much less is known about embryonic development innematodes fromother taxa, especially at themolecular level.We are interestedininsectpathogenicnematodes from the genus Steinernema asmodels of parasitismand symbiosis aswell as a satellitemodel for evolution in comparison toC. elegans. To exploregene expressiondifferences across taxa,we sequencedthe transcriptomesof singleembryosof twoSteinernema species and two Caenorhabditis species at 11 stages during embryonic development and found several interesting features. Our findings show that zygotic transcription initiates at different developmental stages in each species, with the Steinernema species initiating transcription earlier than Caenorhabditis.We found that ortholog expression conservation during development is higher at the later embryonic stages than at the earlier ones. The surprisingly higher conservation of orthologous gene expression in later embryonic stages strongly suggests a funnel-shaped model of embryonic developmental gene expression divergence in nematodes. Thiswork provides novel insight into embryonic development across distantly related nematode species and demonstrates that the mechanisms controlling early development are more diverse than previously thought at the transcriptional level.

Original languageEnglish (US)
Pages (from-to)2681-2696
Number of pages16
JournalGenome biology and evolution
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2017

Bibliographical note

Funding Information:
We would like to thank all of the members of the Mortazavi lab, Dr. Adler Dillman, Dr. Steve Shen, Dr. Olivier Cinquin, Dr. Brandon Gaut, Dr. Ken Cho, Dr. Anne Calof, and Dr. Shaun Hug for helpful discussions. This work was supported by an NIH New Innovator Award to A.M. (NIGMS DP2 GM111100).

Keywords

  • Caenorhabditis
  • Comparative transcriptomics
  • Embryonic development
  • RNA-seq
  • Single-embryo
  • Steinernema

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