Comparative transcriptome and secretome analysis of wood decay fungi postia placenta and phanerochaete chrysosporium

Amber Vanden Wymelenberg, Jill Gaskell, Michael Mozuch, Grzegprz Sabat, John Ralph, Oleksandr Skyba, Shawn D. Mansfield, Robert A. Blanchette, Diego Martinez, Igor Grigoriev, Philip J. Kersten, Dan Cullen

Research output: Contribution to journalArticlepeer-review

174 Scopus citations

Abstract

Cellulose degradation by brown rot fungi, such as Postia placenta, is poorly understood relative to the phylogenetically related white rot basidiomycete, Phanerochaete chrysosporium. To elucidate the number, structure, and regulation of genes involved in lignocellulosic cell wall attack, secretome and transcriptome analyses were performed on both wood decay fungi cultured for 5 days in media containing ball-milled aspen or glucose as the sole carbon source. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), a total of 67 and 79 proteins were identified in the extracellular fluids of P. placenta and P. chrysosporium cultures, respectively. Viewed together with transcript profiles, P. chrysosporium employs an array of extracellular glycosyl hydrolases to simultaneously attack cellulose and hemicelluloses. In contrast, under these same conditions, P. placenta secretes an array of hemicellulases but few potential cellulases. The two species display distinct expression patterns for oxidoreductase-encoding genes. In P. placenta, these patterns are consistent with an extracellular Fenton system and include the upregulation of genes involved in iron acquisition, in the synthesis of low-molecular-weight quiñones, and possibly in redox cycling reactions.

Original languageEnglish (US)
Pages (from-to)3599-3610
Number of pages12
JournalApplied and environmental microbiology
Volume76
Issue number11
DOIs
StatePublished - Jun 2010

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