TY - JOUR
T1 - Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling
AU - Singh, Sudhir P.
AU - Jeet, Raja
AU - Kumar, Jitendra
AU - Shukla, Vishnu
AU - Srivastava, Rakesh
AU - Mantri, Shrikant S.
AU - Tuli, Rakesh
N1 - Funding Information:
The authors thank Executive Director, National Agri-Food Biotechnology Institute (Department of Biotechnology) for facilitating the research work, and Prof. H. S. Dhaliwal of Eternal University for providing seeds. RJ and JK acknowledge ICMR and CSIR, respectively, for providing fellowship.
PY - 2014/11/3
Y1 - 2014/11/3
N2 - Wheat is one of the most important cereal crops in the world. To identify the candidate genes for mineral accumulation, it is important to examine differential transcriptome between wheat genotypes, with contrasting levels of minerals in grains. A transcriptional comparison of developing grains was carried out between two wheat genotypes- Triticum aestivum Cv. WL711 (low grain mineral), and T. aestivum L. IITR26 (high grain mineral), using Affymetrix GeneChip Wheat Genome Array. The study identified a total of 580 probe sets as differentially expressed (with log2 fold change of $2 at p#0.01) between the two genotypes, during grain filling. Transcripts with significant differences in induction or repression between the two genotypes included genes related to metal homeostasis, metal tolerance, lignin and flavonoid biosynthesis, amino acid and protein transport, vacuolar-sorting receptor, aquaporins, and stress responses. Meta-analysis revealed spatial and temporal signatures of a majority of the differentially regulated transcripts.
AB - Wheat is one of the most important cereal crops in the world. To identify the candidate genes for mineral accumulation, it is important to examine differential transcriptome between wheat genotypes, with contrasting levels of minerals in grains. A transcriptional comparison of developing grains was carried out between two wheat genotypes- Triticum aestivum Cv. WL711 (low grain mineral), and T. aestivum L. IITR26 (high grain mineral), using Affymetrix GeneChip Wheat Genome Array. The study identified a total of 580 probe sets as differentially expressed (with log2 fold change of $2 at p#0.01) between the two genotypes, during grain filling. Transcripts with significant differences in induction or repression between the two genotypes included genes related to metal homeostasis, metal tolerance, lignin and flavonoid biosynthesis, amino acid and protein transport, vacuolar-sorting receptor, aquaporins, and stress responses. Meta-analysis revealed spatial and temporal signatures of a majority of the differentially regulated transcripts.
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U2 - 10.1371/journal.pone.0111718
DO - 10.1371/journal.pone.0111718
M3 - Article
C2 - 25364903
AN - SCOPUS:84909970986
SN - 1932-6203
VL - 9
JO - PLoS One
JF - PLoS One
IS - 11
M1 - e111718
ER -