Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling

Sudhir P. Singh, Raja Jeet, Jitendra Kumar, Vishnu Shukla, Rakesh Srivastava, Shrikant S. Mantri, Rakesh Tuli

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Wheat is one of the most important cereal crops in the world. To identify the candidate genes for mineral accumulation, it is important to examine differential transcriptome between wheat genotypes, with contrasting levels of minerals in grains. A transcriptional comparison of developing grains was carried out between two wheat genotypes- Triticum aestivum Cv. WL711 (low grain mineral), and T. aestivum L. IITR26 (high grain mineral), using Affymetrix GeneChip Wheat Genome Array. The study identified a total of 580 probe sets as differentially expressed (with log2 fold change of $2 at p#0.01) between the two genotypes, during grain filling. Transcripts with significant differences in induction or repression between the two genotypes included genes related to metal homeostasis, metal tolerance, lignin and flavonoid biosynthesis, amino acid and protein transport, vacuolar-sorting receptor, aquaporins, and stress responses. Meta-analysis revealed spatial and temporal signatures of a majority of the differentially regulated transcripts.

Original languageEnglish (US)
Article numbere111718
JournalPloS one
Volume9
Issue number11
DOIs
StatePublished - Nov 3 2014
Externally publishedYes

Bibliographical note

Funding Information:
The authors thank Executive Director, National Agri-Food Biotechnology Institute (Department of Biotechnology) for facilitating the research work, and Prof. H. S. Dhaliwal of Eternal University for providing seeds. RJ and JK acknowledge ICMR and CSIR, respectively, for providing fellowship.

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