Abstract
The present study describes potential beneficial and adverse effects of plant-extract synthesized gold nanoparticles (AuNPs) on ethanol toxicity in SH-SY5Y cells. Although kudzu root extract (K), edible-gum extract (G), alone or in combination (KG), reduced Au3+ into AuNPs, the extract's composition and the reaction temperature determined their size (AuNPKG(90<50<37) << AuNPK (90,50<37) < AuNP)G (90<50)) the subscript KG, K, or G is extract identification and numerical vales are reaction temperature in Celsius) and biological properties (AuNPKG (90,50>37) << AuNPK (90,50>37) < AuNPG (90,50)) . The surface of each AuNP contained the extract's active ingredients, that were analyzed and confirmed using laser desorption ionization (LDI)) and low-matrix laser desorption-ionization (LMALDI). AuNPKG-50 was (i) least toxic to SH-SY5Y cells, but most effective in suppressing the adverse effects of ethanol on SH-SY5Y cells, and (ii) more effective than a combination of free kudzu and gum extracts. The beneficial and adverse effects of AuNPs may have been modified by the formation of proteins corona. This study provides a proof of concept for possible application of plant-extract synthesized AuNPs in mitigating ethanol toxicity.
Original language | English (US) |
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Article number | 70 |
Journal | Biomedicines |
Volume | 5 |
Issue number | 4 |
DOIs | |
State | Published - Dec 1 2017 |
Bibliographical note
Publisher Copyright:© 2017 by the author.
Keywords
- Apoptosis
- Cell viability
- Edible gum
- Ethanol
- Gold nanoparticles
- Kudzu root
- Lactate dehydrogenase
- Lipid peroxidase
- Necrosis
- Plant extracts