Comparative survival of ten porcine reproductive and respiratory syndrome virus strains at three temperatures

Angie Quinonez-Munoz, Nader M. Sobhy, Sagar M. Goyal

Research output: Contribution to journalArticlepeer-review


Objective: Comparative survival of 10 strains of porcine reproductive and respiratory syndrome virus (PRRSV) at 3 temperatures. Materials and methods: Strains of PRRSV were propagated in MARC-145 cell line. Aliquots of virus were placed in the bottom of wells on 24-well plates at 100 µL per well. After the virus inoculum was dry, the plates were stored at one of 3 temperatures (4°C, room temperature [22°C-25°C], or 37°C). The surviving virus was eluted at different time points and then titrated. Results: All 10 strains survived for at least 35 days at 4°C but showed variability in percent survival. For example, the percent survival of strains 1-7-4, Lelystad, 1-8-4, VR-2332, 1-4-2, and 1-4-4 MN was greater (0.29%-2.19%) than that of the other 5 strains (0.01%-0.03%). At room temperature, 5 strains (VR-2332, Lelystad, 1-4-4 SD, 1-4-4 MN, and 1-8-4) survived between 3 and 7 days while the other 5 survived for 1 day only. Four of the ten strains (Lelystad, 1-4-4 MN, 1-4-4 SD, and 1-8-4) survived for up to 3 days at 37°C and the remaining 6 strains for 1 day only. The recently emerged variant 1-4-4 L1C was one of the more resistant strains surviving for 7 days at room temperature and 3 days at 37°C. Implications: There were differences in the survival of different PRRSV strains at different temperatures, which should be taken into consideration for designing effective biosecurity practices including disinfection regimens.

Original languageEnglish (US)
Pages (from-to)66-73
Number of pages8
JournalJournal of Swine Health and Production
Issue number2
StatePublished - Mar 2024

Bibliographical note

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© 2024 American Association of Swine Veterinarians. All rights reserved.


  • inactivation
  • porcine reproductive and respiratory syndrome virus variants
  • survivability
  • swine
  • temperature


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