Comparative study of water-soluble polysaccharides isolated from leaves and roots of Isatis indigotica Fort.

Un Hak Pak, Yang Yu, Xin Ning, Chung Hyok Ho, Li Ji, Kevin H. Mayo, Yifa Zhou, Lin Sun

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12 Scopus citations


Water-soluble polysaccharides were isolated from the leaves and roots of Isatis indigotica Fort., and their structural features were studied and compared. One neutral polysaccharide fraction (WFIP-N) and three pectin fractions (WFIP-A-A, WFIP-A-B and WFIP-A-C) were obtained from the leaves, and one neutral polysaccharide fraction (WRIP-N) and two pectin fractions (WRIP-A-A and WRIP-A-B) were obtained from the roots. WFIP-A-B (Mw = 34.6 kDa) and WRIP-A-B (Mw = 29.9 kDa) were the major pectic polysaccharides. Monosaccharide composition, FT-IR, enzymatic hydrolysis, NMR and methylation analysis indicated that both WFIP-A-B and WRIP-A-B are composed of rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II) and homogalacturonan (HG) domains with mass ratios of 1.5:1.0:0.4 and 0.3:1.0:1.7, respectively. WFIP-A-B and WRIP-A-B were found to be rich in RG-I and HG domains, respectively, and mainly contained type II arabinogalactan (AG-II) and α-L-1,5-arabinan side chains, but those in WRIP-A-B were more numerous and longer. Our results provide structural features and differences between these polysaccharides which will help to elucidate their functional differences.

Original languageEnglish (US)
Pages (from-to)642-652
Number of pages11
JournalInternational Journal of Biological Macromolecules
StatePublished - May 1 2022

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China ( 31870796 ), the Scientific and Technologic Foundation of Jilin Province ( 20200201004JC ), the Fundamental Research Funds for the Central Universities ( 2412020ZD001 ) and the Innovation Platform Project of Qinghai province ( 2021-ZJ-T02 ).

Publisher Copyright:
© 2022 Elsevier B.V.


  • Isatis indigotica Fort.
  • Pectin
  • Polysaccharides
  • Structural analysis

PubMed: MeSH publication types

  • Journal Article


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