Comparative study of the toxic effects of fumonisin B1 in rat C6 glioma cells and p53-null mouse embryo fibroblasts

Théophile A. Mobio, Emmanuelle Tavan, Isabelle Baudrimont, Rachid Anane, Maria Rosaria Carratú, Ambaliou Sanni, Messanvi F. Gbeassor, Thomas W. Shier, Jean François Narbonne, Edmond E. Creppy

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The present experiments have been carried out in order to study (comparatively) oxidative stress and its consequences (i.e. modifications of DNA bases and/or DNA fragmentation), cell cycle progression (through two generations) and apoptosis in C6 glioma cells (with normal p53 status) and p53-null mouse embryonic fibroblasts (MEF) after incubation with fumonisin B1 (FB1). Further endpoints, including protein and DNA syntheses as well as cytotoxicity, have been also studied. The results show that FB1 (incubation) produced a significant increase of malondialdehyde (MDA) production (suggestive of lipid peroxidation) which was prevented by antioxidant agents in both cell types. Moreover, FB1 induced a significant and dose-related increase of 8-OH-dG and DNA fragmentation in both C6 glioma and MEF cells. Unlike MEF cells, apoptotic C6 glioma cells were observed after FB1 incubation. Moreover, suppression of cell cycle progression was observed in C6 glioma but not in MEF cell incubated with FB1. The results suggest a possible loss of protective mechanisms (such as p53-dependent apoptosis and cell cycle arrest) in FB1-damaged MEF cells and confirm that cells lacking of mechanisms governed by p53 gene would be more susceptible to neoplastic cascade or mutation following DNA lesions induced by this mycotoxin.

Original languageEnglish (US)
Pages (from-to)65-75
Number of pages11
JournalToxicology
Volume183
Issue number1-3
DOIs
StatePublished - Feb 1 2003

Keywords

  • Antioxidants
  • Apoptosis
  • C6 Glioma cells
  • Cell cycle arrest
  • DNA lesions
  • Fumonisin B
  • p53-Null MEF cells

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