Comparative serological evaluation of avian influenza vaccine in turkeys.

A. Abraham; V. Sivanandan; D. Karunakaran; D. A. Halvorson; J. A. Newman

doi:10.2307/1590980

Comparative serological evaluation of avian influenza vaccine in turkeys.

A. Abraham, V. Sivanandan, D. Karunakaran, D. A. Halvorson, J. A. Newman

Veterinary and Biomedical Sciences

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Abstract

Four- and six-week-old turkeys were vaccinated subcutaneously using avian influenza virus (AIV) A/Duck/613/MN/79 (H4N2) killed oil-emulsion vaccine. Sequential serological tests using agar gel precipitin (AGP), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) for measuring antibodies to AIV were performed up to 4 weeks postvaccination, when birds were challenged intranasally using A/Turkey/MN/80 (H4N2) live AIV. The ELISA was 25 to 1600 times more sensitive than the HI test and was able to detect antibody production earlier than the HI test. All turkeys with an ELISA titer of greater than or equal to 800 were protected against homologous challenge, as measured by virus recovery 3 days postchallenge. Four turkeys out of 20 serologically negative by AGP and HI tests but ELISA-positive were protected.

Original language	English (US)
Pages (from-to)	659-662
Number of pages	4
Journal	Avian diseases
Volume	32
Issue number	4
DOIs	https://doi.org/10.2307/1590980
State	Published - Jan 1 1988

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AU - Sivanandan, V.

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AU - Halvorson, D. A.

AU - Newman, J. A.

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Comparative serological evaluation of avian influenza vaccine in turkeys.

Abstract

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