Comparative pollen–pistil interactions and insect pollination in two Hypoxis species (Hypoxidaceae) in China and North America

Zong Xin Ren, Peter Bernhardt, Retha Edens-Meier, Justin R Zweck, Mike Arduser, Hai Dong Li, Hong Wang

Research output: Contribution to journalArticle

Abstract

We compared the floral ecology and pollen–pistil interactions in Hypoxis hirsuta (L.) Coville from North America and Hypoxis aurea Loureiro from China. Both species are vernal-flowering herbs, with yellow perianths, providing pollen as their only reward. In H. hirsuta, hand self-pollinated, emasculated and bagged control flowers failed to set fruit. When cross-pollinated, 77% of the pistils produced seed-filled capsules while 72% of open, insect-pollinated flowers set seed. Epifluorescence showed that most germinating grains failed to penetrate the stigmatic surface > 24 h after hand self-pollination suggesting early-acting self-incompatibility (SI). The majority of pollinators of H. hirsuta were female bees representing 21 species distributed in four families. Pollen load analyses indicated that most bees were polylectic and carried grains of H. hirsuta mixed with pollen of co-blooming, nectariferous species. In contrast, capsule production of H. aurea was 60% for open, insect-pollinated flowers. Pollen tubes of H. aurea also entered ovules within 24 h, and SI of this species was partial. Evidence of a late-acting rejection in this species following self-pollination included abnormal growth of pollen tubes in styles with fewer tubes penetrating ovules. In contrast, abnormal tube growth was not observed in styles of H. hirsuta. Hypoxis aurea received fewer insect visitations. Pollen was dispersed primarily by hoverflies (Syrphidae) followed by bees. Floral presentation, generalist pollination and two modes of SI found in these two Hypoxis species contribute to our understanding of floral evolution for congeners isolated on different continents.

Original languageEnglish (US)
Pages (from-to)115-126
Number of pages12
JournalPlant Systematics and Evolution
Volume305
Issue number2
DOIs
StatePublished - Feb 1 2019

Fingerprint

Hypoxis
Hypoxidaceae
insect pollination
pollination
pollen
insect
self incompatibility
Apoidea
Syrphidae
China
self-pollination
pollen tubes
flowers
ovules
bee
insects
flower
hands
autogamy
pistil

Keywords

  • Bees
  • Hoverflies
  • Hypoxidaceae
  • Hypoxis
  • Self-incompatibility

Cite this

Comparative pollen–pistil interactions and insect pollination in two Hypoxis species (Hypoxidaceae) in China and North America. / Ren, Zong Xin; Bernhardt, Peter; Edens-Meier, Retha; Zweck, Justin R; Arduser, Mike; Li, Hai Dong; Wang, Hong.

In: Plant Systematics and Evolution, Vol. 305, No. 2, 01.02.2019, p. 115-126.

Research output: Contribution to journalArticle

Ren, Zong Xin ; Bernhardt, Peter ; Edens-Meier, Retha ; Zweck, Justin R ; Arduser, Mike ; Li, Hai Dong ; Wang, Hong. / Comparative pollen–pistil interactions and insect pollination in two Hypoxis species (Hypoxidaceae) in China and North America. In: Plant Systematics and Evolution. 2019 ; Vol. 305, No. 2. pp. 115-126.
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