Comparative pollen–pistil interactions and insect pollination in two Hypoxis species (Hypoxidaceae) in China and North America

Zong Xin Ren, Peter Bernhardt, Retha Edens-Meier, Justin Zweck, Mike Arduser, Hai Dong Li, Hong Wang

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We compared the floral ecology and pollen–pistil interactions in Hypoxis hirsuta (L.) Coville from North America and Hypoxis aurea Loureiro from China. Both species are vernal-flowering herbs, with yellow perianths, providing pollen as their only reward. In H. hirsuta, hand self-pollinated, emasculated and bagged control flowers failed to set fruit. When cross-pollinated, 77% of the pistils produced seed-filled capsules while 72% of open, insect-pollinated flowers set seed. Epifluorescence showed that most germinating grains failed to penetrate the stigmatic surface > 24 h after hand self-pollination suggesting early-acting self-incompatibility (SI). The majority of pollinators of H. hirsuta were female bees representing 21 species distributed in four families. Pollen load analyses indicated that most bees were polylectic and carried grains of H. hirsuta mixed with pollen of co-blooming, nectariferous species. In contrast, capsule production of H. aurea was 60% for open, insect-pollinated flowers. Pollen tubes of H. aurea also entered ovules within 24 h, and SI of this species was partial. Evidence of a late-acting rejection in this species following self-pollination included abnormal growth of pollen tubes in styles with fewer tubes penetrating ovules. In contrast, abnormal tube growth was not observed in styles of H. hirsuta. Hypoxis aurea received fewer insect visitations. Pollen was dispersed primarily by hoverflies (Syrphidae) followed by bees. Floral presentation, generalist pollination and two modes of SI found in these two Hypoxis species contribute to our understanding of floral evolution for congeners isolated on different continents.

Original languageEnglish (US)
Pages (from-to)115-126
Number of pages12
JournalPlant Systematics and Evolution
Volume305
Issue number2
DOIs
StatePublished - Feb 1 2019

Bibliographical note

Funding Information:
We thank Dr. Peter E. Gibbs of the University of St Andrews, UK, for comments and discussions on self-incompatibility; Dr. Huan-Li Xu of China Agricultural University for identification of bee specimens collected on H. aurea and Dr. Kui-Yan Zhang of Institute of Zoology, Chinese Academy of Sciences for identification of hoverflies (Syrphidae). We also thank Ms. Dowen Jocson of St. Louis University for insects measurement. We thank four anonymous reviewers for their detailed comments for improving this manuscript. Dr. Zong-Xin Ren?s work at St. Louis University was funded by Chinese Academy of Sciences. Work of H. aurea was supported by the National Natural Science Foundation of China (No. 31300199) and the Youth Innovation Promotion Association, Chinese Academy of Sciences (2014355).

Funding Information:
Acknowledgements We thank Dr. Peter E. Gibbs of the University of St Andrews, UK, for comments and discussions on self-incompatibility; Dr. Huan-Li Xu of China Agricultural University for identification of bee specimens collected on H. aurea and Dr. Kui-Yan Zhang of Institute of Zoology, Chinese Academy of Sciences for identification of hoverflies (Syrphidae). We also thank Ms. Dowen Jocson of St. Louis University for insects measurement. We thank four anonymous reviewers for their detailed comments for improving this manuscript. Dr. Zong-Xin Ren’s work at St. Louis University was funded by Chinese Academy of Sciences. Work of H. aurea was supported by the National Natural Science Foundation of China (No. 31300199) and the Youth Innovation Promotion Association, Chinese Academy of Sciences (2014355).

Publisher Copyright:
© 2018, Springer-Verlag GmbH Austria, part of Springer Nature.

Keywords

  • Bees
  • Hoverflies
  • Hypoxidaceae
  • Hypoxis
  • Self-incompatibility

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