Comparative phenotypes of peripheral blood and spleen cells from cancer patients

Kathryn E. Cole, Quan P. Ly, Michael A. Hollingsworth, Jesse L. Cox, Ingunn M. Stromnes, James C. Padussis, Jason M. Foster, Luciano M. Vargas, James E. Talmadge

Research output: Contribution to journalArticlepeer-review

Abstract

Patients with resectable tumor, either in the body or the tail of the pancreas, and cancer patients with a primary tumor adjacent to the splenic vasculature frequently undergo a splenectomy as standard of care during resection. The spleen provides an unutilized source of lymphocytes with potential utility for adoptive cellular therapy (ACT). In this report, spleen and peripheral blood (PB) cells from cancer patients were compared to one another and normal PB by flow cytometry with a focus on CD8+ T-cells, memory phenotype, and their relative expression of checkpoint proteins including program death ligand-1 (PD1). PD1 is both an activation marker for T-cells including antigen (Ag) specific responses, as well as a marker of T-cell exhaustion associated with co-expression of other checkpoint molecules such as lymphocyte activating gene-3 (LAG-3) and T-cell immunoglobulin and mucin domain containing-3 (TIM-3). In summary, the spleen is a rich source of CD8+PD1+ T-cells, with an 8-fold higher frequency compared to the PB. These CD8+ T-cells are predominantly central and transitional memory T-cells with associated effector phenotypes and low expression of TIM-3 and LAG-3 with potential utility for ACT”.

Original languageEnglish (US)
Article number106655
JournalInternational Immunopharmacology
Volume85
DOIs
StatePublished - Aug 2020

Bibliographical note

Funding Information:
This work was supported, in whole or in part, by National Institutes of Health Grants (USA) for Specialized Programs of Research Excellence , Grant P50 CA127297 and by the Fred & Pamela Buffett Cancer Center Support Grant P30 CA036727 .

Funding Information:
This work was supported, in whole or in part, by National Institutes of Health Grants (USA) for Specialized Programs of Research Excellence, Grant P50 CA127297 and by the Fred & Pamela Buffett Cancer Center Support Grant P30 CA036727.

Publisher Copyright:
© 2020

PubMed: MeSH publication types

  • Comparative Study
  • Journal Article

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