Comparative Genomics of Barley-Infecting Xanthomonas translucens Shows Overall Genetic Similarity but Globally Distributed Virulence Factor Diversity

Nathaniel Heiden, Veronica Roman-Reyna, Rebecca D. Curland, Ruth Dill-Macky, Jonathan M. Jacobs

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Xanthomonas translucens pv. translucens (Xtt) is a global barley pathogen and a concern for resistance breeding and regulation. Long-read whole genome sequences allow in-depth understanding of pathogen diversity. We have completed long-read PacBio sequencing of two Minnesotan Xtt strains and an in-depth analysis of available Xtt genomes. We found that average nucleotide identity (ANI)-based approaches organize Xtt strains different from the previous standard multilocus sequencing analysis approach.According to ANI,Xtt forms a separate clade from X. translucens pv. undulosa and consists of three main groups which are represented on multiple continents. Some virulence factors, such as 17 Type III-secreted effectors, are highly conserved and offer potential targets for the elicitation of broad resistance. However, there is a high degree of variation in virulence factors, meaning that germplasm should be screened for resistance with a diverse panel of Xtt.

Original languageEnglish (US)
Pages (from-to)2056-2061
Number of pages6
JournalPhytopathology
Volume113
Issue number11
DOIs
StatePublished - Nov 2023

Bibliographical note

Publisher Copyright:
© 2023 American Phytopathological Society. All rights reserved.

Keywords

  • average nucleotide identity
  • barley
  • life identification numbers
  • pathogen distribution
  • phylogeny
  • virulence factors
  • Xanthomonas translucens

PubMed: MeSH publication types

  • Journal Article

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