Comparative genomics and transcriptomics analyses reveal divergent lifestyle features of nematode endoparasitic fungus hirsutella minnesotensis

Yiling Lai, Keke Liu, Xinyu Zhang, Xiaoling Zhang, Kuan Li, Niuniu Wang, Chi Shu, Yunpeng Wu, Chengshu Wang, Kathryn E. Bushley, Meichun Xiang, Xingzhong Liu

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Hirsutella minnesotensis [Ophiocordycipitaceae (Hypocreales, Ascomycota)] is a dominant endoparasitic fungus by using conidia that adhere to and penetrate the secondary stage juveniles of soybean cyst nematode. Its genome was de novo sequenced and compared with five entomopathogenic fungi in the Hypocreales and three nematode-trapping fungi in the Orbiliales (Ascomycota). The genome of H. minnesotensis is 51.4 Mb and encodes 12,702 genes enriched with transposable elements up to 32%. Phylogenomic analysis revealed that H. minnesotensis was diverged from entomopathogenic fungi in Hypocreales. Genome of H. minnesotensis is similar to those of entomopathogenic fungi to have fewer genes encoding lectins for adhesion and glycoside hydrolases for cellulose degradation, but is different from those of nematode-trapping fungi to possess more genes for protein degradation, signal transduction, and secondary metabolism. Those results indicate that H. minnesotensis has evolved different mechanism for nematode endoparasitism compared with nematode-trapping fungi. Transcriptomics analyses for the time-scale parasitism revealed the upregulations of lectins, secreted proteases and the genes for biosynthesis of secondary metabolites that could be putatively involved in host surface adhesion, cuticle degradation, and host manipulation. Genome and transcriptome analyses provided comprehensive understanding of the evolution and lifestyle of nematode endoparasitism.

Original languageEnglish (US)
Pages (from-to)3077-3093
Number of pages17
JournalGenome biology and evolution
Volume6
Issue number11
DOIs
StatePublished - Nov 2014

Bibliographical note

Publisher Copyright:
© The Author(s) 2014.

Keywords

  • Comparative genomics
  • Life strategy
  • Nematode endoparasitic fungus
  • Parasitism mechanism
  • Transcriptome

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