TY - JOUR
T1 - Comparative genome analysis to identify SNPs associated with high oleic acid and elevated protein content in soybean
AU - Kulkarni, Krishnanand P.
AU - Patil, Gunvant
AU - Valliyodan, Babu
AU - Vuong, Tri D.
AU - Shannon, J. Grover
AU - Nguyen, Henry T.
AU - Lee, Jeong Dong
N1 - Funding Information:
This research was supported by the United Soybean Board.
Publisher Copyright:
© 2018 Published by NRC Research Press.
PY - 2018
Y1 - 2018
N2 - The objective of this study was to determine the genetic relationship between the oleic acid and protein content. The genotypes having high oleic acid and elevated protein (HOEP) content were crossed with five elite lines having normal oleic acid and average protein (NOAP) content. The selected accessions were grown at six environments in three different locations and phenotyped for protein, oil, and fatty acid components. The mean protein content of parents, HOEP, and NOAP lines was 34.6%, 38%, and 34.9%, respectively. The oleic acid concentration of parents, HOEP, and NOAP lines was 21.7%, 80.5%, and 20.8%, respectively. The HOEP plants carried both FAD2-1A (S117N) and FAD2-1B (P137R) mutant alleles contributing to the high oleic acid phenotype. Comparative genome analysis using whole-genome resequencing data identified six genes having single nucleotide polymorphism (SNP) significantly associated with the traits analyzed. A single SNP in the putative gene Glyma.10G275800 was associated with the elevated protein content, and palmitic, oleic, and linoleic acids. The genes from the marker intervals of previously identified QTL did not carry SNPs associated with protein content and fatty acid composition in the lines used in this study, indicating that all the genes except Glyma.10G278000 may be the new genes associated with the respective traits.
AB - The objective of this study was to determine the genetic relationship between the oleic acid and protein content. The genotypes having high oleic acid and elevated protein (HOEP) content were crossed with five elite lines having normal oleic acid and average protein (NOAP) content. The selected accessions were grown at six environments in three different locations and phenotyped for protein, oil, and fatty acid components. The mean protein content of parents, HOEP, and NOAP lines was 34.6%, 38%, and 34.9%, respectively. The oleic acid concentration of parents, HOEP, and NOAP lines was 21.7%, 80.5%, and 20.8%, respectively. The HOEP plants carried both FAD2-1A (S117N) and FAD2-1B (P137R) mutant alleles contributing to the high oleic acid phenotype. Comparative genome analysis using whole-genome resequencing data identified six genes having single nucleotide polymorphism (SNP) significantly associated with the traits analyzed. A single SNP in the putative gene Glyma.10G275800 was associated with the elevated protein content, and palmitic, oleic, and linoleic acids. The genes from the marker intervals of previously identified QTL did not carry SNPs associated with protein content and fatty acid composition in the lines used in this study, indicating that all the genes except Glyma.10G278000 may be the new genes associated with the respective traits.
KW - Comparative genome analysis
KW - Elevated protein content
KW - High oleic acid
KW - Soybean
KW - Whole-genome resequencing
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U2 - 10.1139/gen-2017-0158
DO - 10.1139/gen-2017-0158
M3 - Article
C2 - 29365289
AN - SCOPUS:85043322219
SN - 0831-2796
VL - 61
SP - 217
EP - 222
JO - Genome
JF - Genome
IS - 3
ER -