Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli

Justin Courcelle, Arkady Khodursky, Brian Peter, Patrick O. Brown, Philip C. Hanawalt

Research output: Contribution to journalArticlepeer-review

598 Scopus citations

Abstract

The SOS response in UV-irradiated Escherichia coli includes the upregulation of several dozen genes that are negatively regulated by the LexA repressor. Using DNA microarrays containing amplified DNA fragments from 95.5% of all open reading frames identified on the E. coli chromosome, we have examined the changes in gene expression following UV exposure in both wild-type cells and lexA1 mutants, which are unable to induce genes under LexA control. We report here the time courses of expression of the genes surrounding the 26 documented lexA-regulated regions on the E. coli chromosome. We observed 17 additional sites that responded in a lexA-dependent manner and a large number of genes that were upregulated in a lexA-independent manner although upregulation in this manner was generally not more than twofold. In addition, several transcripts were either downregulated or degraded following UV irradiation. These newly identified UV-responsive genes are discussed with respect to their possible roles in cellular recovery following exposure to UV irradiation.

Original languageEnglish (US)
Pages (from-to)41-64
Number of pages24
JournalGenetics
Volume158
Issue number1
StatePublished - 2001
Externally publishedYes

Fingerprint Dive into the research topics of 'Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli'. Together they form a unique fingerprint.

Cite this