Comparative analysis of Streptococcus suis genomes identifies novel candidate virulence-associated genes in North American isolates

April A Estrada, Marcelo Gottschalk, Connie J. Gebhart, Douglas Marthaler

Research output: Contribution to journalArticlepeer-review

Abstract

Streptococcus suis is a significant economic and welfare concern in the swine industry. Pan-genome analysis provides an in-silico approach for the discovery of genes involved in pathogenesis in bacterial pathogens. In this study, we performed pan-genome analysis of 208 S. suis isolates classified into the pathogenic, possibly opportunistic, and commensal pathotypes to identify novel candidate virulence-associated genes (VAGs) of S. suis. Using chi-square tests and LASSO regression models, three accessory pan-genes corresponding to S. suis strain P1/7 markers SSU_RS09525, SSU_RS09155, and SSU_RS03100 (>95% identity) were identified as having a significant association with the pathogenic pathotype. The proposed novel SSU_RS09525 + /SSU_RS09155 + /SSU_RS03100 + genotype identified 96% of the pathogenic pathotype strains, suggesting a novel genotyping scheme for predicting the pathogenicity of S. suis isolates in North America. In addition, mobile genetic elements carrying antimicrobial resistance genes (ARGs) and VAGs were identified but did not appear to play a major role in the spread of ARGs and VAGs.

Original languageEnglish (US)
Pages (from-to)23
Number of pages1
JournalVeterinary research
Volume53
Issue number1
DOIs
StatePublished - Mar 18 2022

Bibliographical note

Publisher Copyright:
© 2022. The Author(s).

Keywords

  • Streptococcus suis
  • comparative genomics
  • pathotype
  • virulence-associated genes (VAGs)
  • Genome, Bacterial
  • Animals
  • Swine
  • Streptococcus suis/genetics
  • Genotype
  • Virulence/genetics
  • Swine Diseases/microbiology

PubMed: MeSH publication types

  • Journal Article

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