Abstract
Trichophyton tonsurans (TT) and Trichophyton equinum (TE) are two closely related dermatophytes with very different host preferences. This study was designed to examine the genetic and transcript level variations of secreted enzymes between TT and TE. Thirty-one genes representing 10 gene families were selected for comparison and complete genomic and cDNA sequences were elucidated. Sequence analyses of the selected genes identified 104 polymorphisms between the two dermatophytes, 37 of which are expected to encode changes in their polypeptide sequence. Quantitative RT-PCR was used to examine the differences in levels of transcript between TT and TE grown over 14. d in aqueous keratin medium. Differences in transcript expression between TT and TE were gene specific and ranged from 1.1-fold to 33-fold. Intra-specific variability across all genes ranged from 41. % to 250. %. Despite their overall genetic similarity, TT and TE exhibit a moderate degree of variability in the genomic make-up of their secreted enzymes and the extent to which they are transcribed when grown in an aqueous keratin medium. Such differences may contribute to how these genetically similar organisms have adapted to infect divergent host organisms.
Original language | English (US) |
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Pages (from-to) | 429-437 |
Number of pages | 9 |
Journal | Fungal Biology |
Volume | 114 |
Issue number | 5-6 |
DOIs | |
State | Published - May 2010 |
Externally published | Yes |
Bibliographical note
Funding Information:This study was supported by a grant from the Kenneth L. and Eva S. Smith foundation.
Keywords
- Dermatophyte
- Protease
- Secreted
- Transcript
- Trichophyton