TY - JOUR
T1 - Comparative analysis of 18 sex pheromone plasmids from Enterococcus faecalis
T2 - Detection of a new insertion element on pPD1 and implications for the evolution of this plasmid family
AU - Hirt, Helmut
AU - Wirth, Reinhard
AU - Muscholl, Albrecht
PY - 1996
Y1 - 1996
N2 - A new IS element, IS1062, related to the enterococcal IS elements IS6770 and IS1252, was detected in the 3'-terminus of the surface exclusion gene, sep1, of sex pheromone plasmid pPD1 in Enterococcus faecalis. pPD1-bearing cells lack the surface exclusion function, probably as a consequence of this insertion. Analysis of pAD1 and pPD1 sequences (7.5 kb and 2.7 kb, respectively) downstream of their aggregation substance genes revealed no similarity in these DNA regions. Detailed DNA/DNA hybridization studies using DNA probes specific for various pAD1-encoded genes needed for plasmid transfer indicated that the sex pheromone plasmids have evolved by repeated recombination and insertion of diverse transposable elements which presumably account for recent acquisition of antibiotic resistances.
AB - A new IS element, IS1062, related to the enterococcal IS elements IS6770 and IS1252, was detected in the 3'-terminus of the surface exclusion gene, sep1, of sex pheromone plasmid pPD1 in Enterococcus faecalis. pPD1-bearing cells lack the surface exclusion function, probably as a consequence of this insertion. Analysis of pAD1 and pPD1 sequences (7.5 kb and 2.7 kb, respectively) downstream of their aggregation substance genes revealed no similarity in these DNA regions. Detailed DNA/DNA hybridization studies using DNA probes specific for various pAD1-encoded genes needed for plasmid transfer indicated that the sex pheromone plasmids have evolved by repeated recombination and insertion of diverse transposable elements which presumably account for recent acquisition of antibiotic resistances.
KW - Enterococcus faecalis
KW - Insertion element IS1062
KW - Plasmid evolution
KW - Sex pheromone plasmids
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U2 - 10.1007/s004380050272
DO - 10.1007/s004380050272
M3 - Article
C2 - 8917306
AN - SCOPUS:0029911897
SN - 0026-8925
VL - 252
SP - 640
EP - 647
JO - Molecular and General Genetics
JF - Molecular and General Genetics
IS - 6
ER -