Community structure of subsurface biofilms in the thermal sulfidic caves of Acquasanta Terme, Italy

Daniel S Jones, D. J. Tobler, I. Schaperdoth, M. Mainiero, J. L. Macalady

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We performed a microbial community analysis of biofilms inhabiting thermal (35 to 50°C) waters more than 60 m below the ground surface near Acquasanta Terme, Italy. The groundwater hosting the biofilms has 400 to 830 μM sulfide, <10 μM O2, pH of 6.3 to 6.7, and specific conductivity of 8,500 to 10,500 μS/cm. Based on the results of 16S rRNA gene cloning and fluorescent in situ hybridization (FISH), the biofilms have low species richness, and lithoautotrophic (or possibly mixotrophic) Gamma-and Epsilonproteobacteria are the principle biofilm architects. Deltaproteobacteria sequences retrieved from the biofilms have <90% 16S rRNA similarity to their closest relatives in public databases and may represent novel sulfate-reducing bacteria. The Acquasanta biofilms share few species in common with Frasassi cave biofilms (13°C, 80 km distant) but have a similar community structure, with representatives in the same major clades. The ecological success of Sulfurovumales-group Epsilonproteobacteria in the Acquasanta biofilms is consistent with previous observations of their dominance in sulfidic cave waters with turbulent water flow and high dissolved sulfide/oxygen ratios.

Original languageEnglish (US)
Pages (from-to)5902-5910
Number of pages9
JournalApplied and environmental microbiology
Volume76
Issue number17
DOIs
StatePublished - Sep 1 2010

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Biofilms
caves
biofilm
Italy
cave
community structure
Hot Temperature
heat
Epsilonproteobacteria
epsilon-Proteobacteria
Sulfides
sulfides
Water
Deltaproteobacteria
sulfide
ribosomal RNA
delta-Proteobacteria
sulfate-reducing bacteria
Groundwater
sulfate-reducing bacterium

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Community structure of subsurface biofilms in the thermal sulfidic caves of Acquasanta Terme, Italy. / Jones, Daniel S; Tobler, D. J.; Schaperdoth, I.; Mainiero, M.; Macalady, J. L.

In: Applied and environmental microbiology, Vol. 76, No. 17, 01.09.2010, p. 5902-5910.

Research output: Contribution to journalArticle

Jones, Daniel S ; Tobler, D. J. ; Schaperdoth, I. ; Mainiero, M. ; Macalady, J. L. / Community structure of subsurface biofilms in the thermal sulfidic caves of Acquasanta Terme, Italy. In: Applied and environmental microbiology. 2010 ; Vol. 76, No. 17. pp. 5902-5910.
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