Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties

James M. Rae; Susan J. Ramus; Mark Waltham; Jane E. Armes; Ian G. Campbell; Robert Clarke; Robert J. Barndt; Michael D. Johnson; Erik W. Thompson

doi:10.1007/s10585-004-3759-1

Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties

James M. Rae
, Susan J. Ramus
, Mark Waltham
, Jane E. Armes
, Ian G. Campbell
, Robert Clarke
, Robert J. Barndt
, Michael D. Johnson
, Erik W. Thompson

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). Gene expression profile analy-sis confirmed common expression of genes among different MDA-MB-435-LCC cell stocks, and identified some unique gene products in MDA-MB-435-PMCC cells. RT-PCR analysis confirmed the expression of the melanoma marker tyrosinase across multiple MDA-MB-435 cell stocks. Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift.

Original language	English (US)
Pages (from-to)	543-552
Number of pages	10
Journal	Clinical and Experimental Metastasis
Volume	21
Issue number	6
DOIs	https://doi.org/10.1007/s10585-004-3759-1
State	Published - Oct 2004
Externally published	Yes

Bibliographical note

Funding Information:
The authors acknowledge the collegiality of Dr. Janet Price, MD Anderson Cancer Center for comments and assistance with this manuscript. Fleur Hammet, Melanie de Silva, Anne-Marie Hutchins from the Molecular Pathology laboratory of the Victorian Breast Cancer Research Consortium are thanked for their assistance. The authors also wish to thank Dr Dominic A. Scu-diero of the National Institutes of Health for DNA and RNA from MDA-MB-435-DTP cells. This work was primarily supported by the Victorian Breast Cancer Research Consortium and grants from the Breast Cancer Research Foundation (N003173), US-DOD (BC 021320) and NIH (R21 CA87244-01). We thank the Microscopy and Imaging, Tissue Culture, Biostatistics, Research Computing, and Macromolecular Shared Resources of the Lombardi Cancer Center, which are partially supported by PHS grant NIH 1P30-CA-51008 (Cancer Center Support Grant) to Lombardi Cancer Center.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Breast cancer
Cell lines
Chromosomal number
MDA-MB-435
Melanoma
Microsatellite analysis
Tyrosinase

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10.1007/s10585-004-3759-1

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title = "Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties",

abstract = "Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). Gene expression profile analy-sis confirmed common expression of genes among different MDA-MB-435-LCC cell stocks, and identified some unique gene products in MDA-MB-435-PMCC cells. RT-PCR analysis confirmed the expression of the melanoma marker tyrosinase across multiple MDA-MB-435 cell stocks. Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift.",

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author = "Rae, \{James M.\} and Ramus, \{Susan J.\} and Mark Waltham and Armes, \{Jane E.\} and Campbell, \{Ian G.\} and Robert Clarke and Barndt, \{Robert J.\} and Johnson, \{Michael D.\} and Thompson, \{Erik W.\}",

note = "Funding Information: The authors acknowledge the collegiality of Dr. Janet Price, MD Anderson Cancer Center for comments and assistance with this manuscript. Fleur Hammet, Melanie de Silva, Anne-Marie Hutchins from the Molecular Pathology laboratory of the Victorian Breast Cancer Research Consortium are thanked for their assistance. The authors also wish to thank Dr Dominic A. Scu-diero of the National Institutes of Health for DNA and RNA from MDA-MB-435-DTP cells. This work was primarily supported by the Victorian Breast Cancer Research Consortium and grants from the Breast Cancer Research Foundation (N003173), US-DOD (BC 021320) and NIH (R21 CA87244-01). We thank the Microscopy and Imaging, Tissue Culture, Biostatistics, Research Computing, and Macromolecular Shared Resources of the Lombardi Cancer Center, which are partially supported by PHS grant NIH 1P30-CA-51008 (Cancer Center Support Grant) to Lombardi Cancer Center.",

year = "2004",

month = oct,

doi = "10.1007/s10585-004-3759-1",

language = "English (US)",

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AU - Rae, James M.

AU - Ramus, Susan J.

AU - Waltham, Mark

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AU - Campbell, Ian G.

AU - Clarke, Robert

AU - Barndt, Robert J.

AU - Johnson, Michael D.

AU - Thompson, Erik W.

N1 - Funding Information: The authors acknowledge the collegiality of Dr. Janet Price, MD Anderson Cancer Center for comments and assistance with this manuscript. Fleur Hammet, Melanie de Silva, Anne-Marie Hutchins from the Molecular Pathology laboratory of the Victorian Breast Cancer Research Consortium are thanked for their assistance. The authors also wish to thank Dr Dominic A. Scu-diero of the National Institutes of Health for DNA and RNA from MDA-MB-435-DTP cells. This work was primarily supported by the Victorian Breast Cancer Research Consortium and grants from the Breast Cancer Research Foundation (N003173), US-DOD (BC 021320) and NIH (R21 CA87244-01). We thank the Microscopy and Imaging, Tissue Culture, Biostatistics, Research Computing, and Macromolecular Shared Resources of the Lombardi Cancer Center, which are partially supported by PHS grant NIH 1P30-CA-51008 (Cancer Center Support Grant) to Lombardi Cancer Center.

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N2 - Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). Gene expression profile analy-sis confirmed common expression of genes among different MDA-MB-435-LCC cell stocks, and identified some unique gene products in MDA-MB-435-PMCC cells. RT-PCR analysis confirmed the expression of the melanoma marker tyrosinase across multiple MDA-MB-435 cell stocks. Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift.

AB - Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). Gene expression profile analy-sis confirmed common expression of genes among different MDA-MB-435-LCC cell stocks, and identified some unique gene products in MDA-MB-435-PMCC cells. RT-PCR analysis confirmed the expression of the melanoma marker tyrosinase across multiple MDA-MB-435 cell stocks. Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift.

KW - Breast cancer

KW - Cell lines

KW - Chromosomal number

KW - MDA-MB-435

KW - Melanoma

KW - Microsatellite analysis

KW - Tyrosinase

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UR - https://www.scopus.com/pages/publications/12544252451#tab=citedBy

U2 - 10.1007/s10585-004-3759-1

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JO - Clinical and Experimental Metastasis

JF - Clinical and Experimental Metastasis

IS - 6

ER -

Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties

Abstract

Bibliographical note

UN SDGs

Keywords

Publisher link

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